Analytical Services Core

分析核心B为了解结直肠癌的“系统生物学”方法提供了支持。这一方法包括协调组织的收集、加工和分发。此外，Analytic Core还进行各种分子形态分析，包括常规组织学、免疫组织化学、原位杂交、激光捕获显微切割、RT-PCR、数字摄影。该核心还促进了利用斑马鱼作为功能基因组学模型系统来研究可能导致结肠癌的分子途径的基因。CORE B提供三项主要服务：(1)人类结肠组织标本的收集和存储；(2)人类和小鼠结肠组织的表征；(3)斑马鱼肠道发育相关基因的检测。 1.人结肠组织标本收集。Analytic Gore B与亨斯迈癌症研究所组织获取和分配核心和核心C合作，为结肠镜检查或参与医院手术切除的组织提供集中收集和分配。两组受试者均预期获得知情同意。 2.人和小鼠结肠组织的生物学特性为了最大限度地利用来自组织样本的试剂并创建统一的试剂，Analytcal Core B从人类或小鼠的正常结肠、结肠腺瘤和结肠癌中分离出DNA和RNA。该核心应项目负责人的要求存储和分发RNA和DNA。此外，该核心还从来自结肠癌、息肉和正常组织的RNA中生成和维护cDNA文库。 3.斑马鱼功能基因组资源。斑马鱼代表了一个易于处理的模型系统，用于快速操纵和识别肠细胞分化所需的特定信号通路。鉴于斑马鱼作为模型系统的属性。Core B通过过表达或吗啡抑制来提供基因调控服务，以支持我们的系统生物学努力，并有效地检查肠道分化的遗传途径。

Analytic Core B provides support for a "systems biology" approach to understanding colorectal cancer. This approach includes coordinating tissue collection, processing and distribution. In addition, the Analytic Core performs a variety of molecular morphological analyses including routine histology, immunohistochemistry, in situ hybridization, laser capture microdissection, RT-PCR, digital photography. The core also facilitates the study of genes that may underlie the molecular pathways that lead to colon cancer using zebrafish as a functional genomics model system. Core B offers three main services: (1) collection and storage of human colon tissue specimens; (2) characterization of human and murine colon tissues and (3) examination of relevant genes in zebrafish intestinal development. 1. Collection of Human Colon Tissue Specimens. Analytical Gore B works with the Huntsman Cancer Institute Tissue Acquistion and Distribution Core and Core C to provide centralized collection and distribution of tissues taken during colonoscopy or from surgical resections performed at participating hospitals. Informed consent is obtained prospectively for both groups of subjects. 2. Characterization of Human and Murine Colon Tissues. To maximize utilization of reagents derived from tissue specimens and to create uniform reagents, Analytcal Core B isolates DNAs and RNAs from normal colon, colon adenomas, and colon carcinomas taken from either humans or mice. This core stores and distributes RNA and DNA at the request of the project leaders. In addition, the core generates and maintains cDNA libraries from RNA taken from colon tumors, polyps and normal tissue. 3. Zebrafish Functional Genomics Resource. Zebrafish represent a tractable model system for quickly manipulating and identifying specific signaling pathways required for enterocyte differentiation. In view of the attributes of zebrafish as a model system. Core B provides gene maniplulation services either though overexpression or morpholino knock down to support our systems biology effort and to efficiently examine genetic pathways of intestinal differentiation.