Analytical Services Core

分析服务核心

• 批准号: 8234103
• 负责人: DAVID A JONES
• 金额: $ 24.38万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8234103
• 关键词: Biological Models; Clinical; Collection; Colon; Colon Carcinoma; Colonic Adenoma; Colonic Neoplasms; Colonoscopy; Colorectal Cancer; DNA; Development; Digital Photography; Drug or chemical Tissue Distribution; Enterocytes; Excision; Genes; Genetic; Goals; Histology; Hospitals; Human; Immunohistochemistry; In Situ Hybridization; Informed Consent; Institutes; Intestines; Lead; Malignant Neoplasms; Measures; Molecular; Mus; Mutation; Normal tissue morphology; Operative Surgical Procedures; Pathway interactions; Polyps; Preventive; Process; RNA; Reagent; Resources; Reverse Transcriptase Polymerase Chain Reaction; Services; Signal Pathway; Specimen; Systems Biology; Tissue Banks; Tissues; Work; Zebrafish; cDNA Library; functional genomics; intestinal epithelium; knock-down; laser capture microdissection; overexpression; prevent

Analytic Core B provides support for a "systems biology" approach to understanding colorectal cancer. This approach includes coordinating tissue collection, processing and distribution. In addition, the Analytic Core performs a variety of molecular morphological analyses including routine histology, immunohistochemistry, in situ hybridization, laser capture microdissection, RT-PCR, digital photography. The core also facilitates the study of genes that may underlie the molecular pathways that lead to colon cancer using zebrafish as a functional genomics model system. Core B offers three main services: (1) collection and storage of human colon tissue specimens; (2) characterization of human and murine colon tissues and (3) examination of relevant genes in zebrafish intestinal development. 1. Collection of Human Colon Tissue Specimens. Analytical Gore B works with the Huntsman Cancer Institute Tissue Acquistion and Distribution Core and Core C to provide centralized collection and distribution of tissues taken during colonoscopy or from surgical resections performed at participating hospitals. Informed consent is obtained prospectively for both groups of subjects. 2. Characterization of Human and Murine Colon Tissues. To maximize utilization of reagents derived from tissue specimens and to create uniform reagents, Analytcal Core B isolates DNAs and RNAs from normal colon, colon adenomas, and colon carcinomas taken from either humans or mice. This core stores and distributes RNA and DNA at the request of the project leaders. In addition, the core generates and maintains cDNA libraries from RNA taken from colon tumors, polyps and normal tissue. 3. Zebrafish Functional Genomics Resource. Zebrafish represent a tractable model system for quickly manipulating and identifying specific signaling pathways required for enterocyte differentiation. In view of the attributes of zebrafish as a model system. Core B provides gene maniplulation services either though overexpression or morpholino knock down to support our systems biology effort and to efficiently examine genetic pathways of intestinal differentiation.

分析核心B为理解结直肠癌的“系统生物学”方法提供支持。这种方法包括协调组织收集、处理和分发。此外，Analytic Core还可进行多种分子形态学分析，包括常规组织学、免疫组织化学、原位杂交、激光捕获显微切割、RT-PCR和数码摄影。该核心还有助于研究可能导致结肠癌的分子途径的基因，使用斑马鱼作为功能基因组学模型系统。Core B主要提供三项服务：（1）人类结肠组织标本的采集和储存;（2）人类和小鼠结肠组织的表征;（3）斑马鱼肠道发育相关基因的检测。 1.人结肠组织标本的采集。Analytical戈尔B与亨斯迈癌症研究所组织采集和分配核心和核心C合作，提供结肠镜检查期间或在参与医院进行的手术切除中采集的组织的集中收集和分配。前瞻性地获得两组受试者的知情同意书。 2.人和鼠结肠组织的表征。为了最大限度地利用来自组织样本的试剂并创建统一的试剂，Analytcal Core B从取自人或小鼠的正常结肠、结肠腺瘤和结肠癌中分离DNA和RNA。该核心应项目负责人的要求储存和分配RNA和DNA。此外，该核心从结肠肿瘤、息肉和正常组织中提取的RNA生成并维持cDNA文库。 3.斑马鱼功能基因组学资源。斑马鱼代表了一个易于处理的模型系统，用于快速操纵和识别肠上皮细胞分化所需的特定信号通路。鉴于斑马鱼的属性作为模式系统。核心B通过过表达或吗啉代敲除提供基因操纵服务，以支持我们的系统生物学工作，并有效地检查肠分化的遗传途径。