EFFECTS OF HYALURONAN ON NEURAL STEM CELL HOMING AND DIFFERENTIATION

透明质酸对神经干细胞归巢和分化的影响

• 批准号: 8357755
• 负责人: Larry S. Sherman
• 金额: $ 3.63万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357755
• 关键词: Adult; Apoptosis; Axon; Blood; Blood Vessels; CD44 Antigens; CD44 gene; Cell Maturation; Cells; Data; Experimental Autoimmune Encephalomyelitis; Funding; Glycoproteins; Grant; Homing; Hyaluronan; Inflammatory; Injection of therapeutic agent; Lesion; Mediator of activation protein; Molecular Weight; Mus; National Center for Research Resources; Oligodendroglia; Primates; Principal Investigator; Production; Research; Research Infrastructure; Resources; Source; Stem cells; T-Lymphocyte; Testing; Undifferentiated; United States National Institutes of Health; cost; cytokine; dysmyelination; nerve stem cell; stem cell differentiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Systemic injection of adult neural stem cells (aNSCs) into mice with experimental autoimmune encephalomyelitis (EAE) can provide a source of new oligodendrocytes that can remyelinate demyelinated or dysmyelinated axons. However, systemically-injected aNSCs often fail to differentiate and remain undifferentiated in peri-vascular domains of demyelinated lesions where they promote apoptosis of blood-born CNS-infiltrating encephalitogenic T cells. We discovered that a high molecular weight form of hyaluronan (HA), accumulates in demyelinated lesions and inhibits oligodendrocyte progenitor cell maturation. The major receptor for HA is the CD44 transmembrane glycoprotein. Our preliminary data suggest that HA and CD44 regulate functions relevant to how aNSCs behave in demyelinated lesions, including NSC differentiation and production of pro-inflammatory cytokines. We propose that CD44 and HA are critical mediators of aNSC recruitment to demyelinated lesions and that HA, at least, can regulate whether NSCs differentiate into myelinating cells or fail to differentiate and promote T cell apoptosis at perivascular domains where HA accumulates. In this study, we aim to test the hypotheses: (1) That high molecular weight HA inhibits aNSC differentiation; (2) That NSC homing to EAE lesions depends on interactions between CD44 and HA; and (3) That HA regulates the immunomodulatory activities of NSC.

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