EVALUTATION OF HUMAN STEM CELL ENGRAFTMENT TO SHIVERER MICE

人类干细胞植入颤抖小鼠的评估

• 批准号: 8357890
• 负责人: Larry S. Sherman
• 金额: $ 3.63万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357890
• 关键词: Action Potentials; Brain; Cell Culture System; Core Facility; Corpus Callosum; Demyelinating Diseases; Electrophysiology (science); Engraftment; Funding; Grant; Human; Laboratories; Mus; Myelin; National Center for Research Resources; Neurosciences; Oligodendroglia; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Shipping; Ships; Shivering; Source; Staining method; Stains; Stem cell transplant; Stem cells; Testing; Tissues; Transplantation; United States National Institutes of Health; cost; human stem cells; nerve stem cell; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is a collaborative effort between the Sherman lab and Stem Cells Inc. focused on testing whether human neural stem cells transplanted into shiverer mice can promote functional remyelination. Mice will be transplanted in the laboratories at Stem Cells Inc. then shipped to the Sherman lab. The corpus callosum will then be dissected from the brains of mice and tested for compound action potentials (CAPs) in the electrophysiology core facility in the Division of Neuroscience. Following recordings, tissues will be stained for the presence of oligodendrocytes and myelin. These studies will demonstrate the efficacy of a novel human neural stem cell culture system for the treatment of demyelinating diseases.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该项目是谢尔曼实验室和干细胞公司之间的合作项目，重点是测试移植到颤抖小鼠体内的人类神经干细胞是否可以促进功能性髓鞘再生。 小鼠将被移植到干细胞公司的实验室，然后运往谢尔曼实验室。 然后将从小鼠大脑中切下胼胝体，并在神经科学部的电生理学核心设施中测试复合动作电位（CAP）。记录后，将对组织进行染色以确定少突胶质细胞和髓磷脂的存在。 这些研究将证明新型人类神经干细胞培养系统治疗脱髓鞘疾病的功效。