ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS MACAQUE COLONIES

无特定病原体的恒河猴群体的建立

• 批准号: 8357737
• 负责人: Cynthia J Doane
• 金额: $ 19.49万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357737
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Alleles; Animals; Assisted Reproductive Technology; Breeding; Cells; Complex; Female; Funding; Future; Genotype; Grant; HIV; Haplotypes; Housing; Immune response; Macaca mulatta; Mediating; Modeling; National Center for Research Resources; Oregon; Pathogenesis; Primates; Principal Investigator; Production; Research; Research Infrastructure; Resources; SIV; Shelter facility; Source; Specific Pathogen Frees; United States National Institutes of Health; Vaccines; Variant; Virus; Virus Diseases; cost; genetic pedigree; germ free condition; immune function; juvenile animal; male; mature animal; offspring; prevent; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The projected need for Indian rhesus macaques for AIDS-related research exceeds availability from current domestic breeding programs and there is an urgent need to expand breeding programs for future AIDS vaccine and pathogenesis studies. Rhesus macaques with defined major histocompatability complex (MHC) genotypes and known pedigree are becoming increasingly important for research to understand biologic variation in host immune responses and its effects on vaccine strategies and the pathogenesis of AIDS. The objective of this project is to expand the Oregon National Primate Research Center's specific pathogen-free (SPF) Indian rhesus macaque resource and sufficiently characterize their MHC haplotype to permit selected pedigree breeding for MHC class I alleles useful in AIDS research. Specific aims for accomplishing this objective include intensively managing a subpopulation of the Center's SPF Indian rhesus macaque breeding colony to maximize production of genetically diverse females to expand the breeding capacity of the colony. The breeding colony is typed for ten MHC alleles and managed for the production of MHC- defined offspring of known parentage. Both selective breeding of MHC-typed animals and assisted reproduction technology are used to enhance production of future breeder males that are homozygous for the MAMU-A*01 and other alleles important for assessing virus-specific cell-mediated immune function in simian immunodeficiency virus vaccine models for preventing AIDS virus infection. The colony is maintained in state-of-the-art sheltered field cage housing and is comprised of approximately 660 adult and juvenile animals and produces approximately 130 offspring annually.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 预计印度恒河猴用于艾滋病相关研究的需求超过 目前国内育种计划的可用性，迫切需要 扩大未来艾滋病疫苗和发病机制研究的育种计划。恒河 具有确定的主要组织相容性复合体（MHC）基因型的猕猴， 已知谱系对于研究了解变得越来越重要 宿主免疫应答的生物学变异及其对疫苗策略的影响， 艾滋病的发病机制。该项目的目标是扩大俄勒冈州 国家灵长类动物研究中心的无特定病原体（SPF）印度恒河猴 猕猴资源，并充分表征其MHC单倍型， 选择用于AIDS研究的MHC I类等位基因的系谱育种。具体 实现这一目标的目标包括集中管理 该中心的SPF印度恒河猴繁殖群，以最大限度地生产 遗传多样性的雌性来扩大殖民地的繁殖能力。的 对繁殖集落进行10种MHC等位基因的分型，并进行管理以产生MHC- 已知亲子关系的后代。MHC型动物的选择性繁殖 和辅助生殖技术，以提高生产的未来 对于MAMU-A*01和其他重要等位基因是纯合的育种雄性 用于评估猴免疫缺陷中病毒特异性细胞介导的免疫功能 预防艾滋病病毒感染的病毒疫苗模型。殖民地维持在 国家的最先进的庇护场笼舍，并包括约660 成年和幼年动物，每年产生约130只后代。