The Natural History of Pediatric Onset Cutaneous and Systemic Mastocytosis

儿童发病的皮肤和系统性肥大细胞增多症的自然史

• 批准号: 8556032
• 负责人: melody c carter
• 金额: $ 21.5万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8556032
• 关键词: 3' Untranslated Regions; Affect; Binding Sites; Biological Markers; Birth; Bone marrow biopsy; Cell Line; Child; Childhood; Classification; Codon Nucleotides; Collaborations; Cutaneous Mastocytosis; Disease; Disease Management; Disease Progression; Disease regression; Enrollment; Family; Family member; Goals; Lead; Mediating; Messenger RNA; MicroRNAs; Mutation; Natural History; Patients; Proteins; Receptor Protein-Tyrosine Kinases; Regulation; Regulatory Pathway; Research; Serum; Signal Transduction; Systemic Mastocytosis; Transcription Factor 3; Tryptase; aged; genetic association; improved; mast cell; mastocytosis; microphthalmia-associated transcription factor; novel

In our on-going studies, we have found that serum tryptase appears to reflect extent of disease and to serve as a biomarker of both disease regression and progression. We are also increasing our enrollment of families with more than one affected family member in order to investigate genetic associations. We are also documenting bone marrow biopsy findings that may similarly be used to predict disease progression and may lead the way to an improved classification of pediatric onset mastocytosis. Activating mutations in codon D816 of the tyrosine kinase receptor, KIT, are found in the majority of patients with systemic mastocytosis. In a collaboration with Dr. Takemoto and his research group, we found that the transcription factor, microphthalmia-associated transcription factor (MITF), is highly expressed in bone marrow biopsies from patients with systemic mastocytosis and activating c-KIT mutations and that KIT signaling markedly up-regulates MITF protein. MITF mRNA levels did not change significantly with KIT signaling which suggested posttranscriptional regulation. An array screen from mast cells identified miR-539 and miR-381 as being down-regulated by KIT signaling and that they repressed MITF expression through conserved miRNA binding sites in the MITF 3'-untranslated region. Forced expression of these miRNAs suppressed MITF protein and inhibited colony-forming capacity of mastocytosis cell lines, supporting the conclusion that a novel regulatory pathway exists between 2 critical mast cell factors, KIT and MITF which is mediated by miRNAs.

在我们正在进行的研究中，我们发现血清类胰蛋白酶似乎反映了疾病的程度，并作为疾病消退和进展的生物标志物。我们还增加了有一个以上受影响家庭成员的家庭的登记，以调查遗传关联。 我们还记录了骨髓活检结果，这些结果同样可以用于预测疾病进展，并可能导致儿童肥大细胞增多症的分类改进。 酪氨酸激酶受体（KIT）密码子D816的激活突变见于大多数系统性肥大细胞增多症患者。在与Takemoto博士及其研究小组的合作中，我们发现转录因子，小眼症相关转录因子（MITF），在系统性肥大细胞增多症和激活c-KIT突变患者的骨髓活检中高度表达，并且KIT信号显着上调MITF蛋白。MITF mRNA水平没有显着改变与KIT信号，这表明转录后调节。来自肥大细胞的阵列筛选鉴定了miR-539和miR-381被KIT信号转导下调，并且它们通过MITF 3 '-非翻译区中的保守miRNA结合位点抑制MITF表达。这些miRNAs的强制表达抑制了肥大细胞增生症细胞系的MITF蛋白和集落形成能力，支持了在两个关键肥大细胞因子KIT和MITF之间存在由miRNAs介导的新的调节途径的结论。