Diagnosis of Ovarian Cancer by Confocal Microendoscopy

本研究的目的是进一步开发和临床验证一种实时多光谱 共聚焦显微内窥镜用于卵巢癌的体内诊断。共焦 显微内窥镜是一种用于在细胞水平上观察组织的新型仪器， 显示出进行光学活检的巨大前景。共聚焦显微内窥镜有可能 为医生提供组织的即时评估并测量更大的区域 减少了传统组织提取活检的取样误差。共焦 在先前的资助期间，构建了微型腹腔镜系统并在人体内进行了测试 期这项工作的一个具体目标是现在验证该系统，并表明它可以用于 在腹腔镜手术中检测卵巢癌。工作的其他目标 是将该仪器的使用范围扩大到检测腹膜种植体的卵巢癌 并开发和测试能够在腹腔内成像的系统。 输卵管进一步的技术发展旨在改善共焦成像 性能和增加光学相干断层扫描作为补充成像模态。 新仪器将把两种成像模式整合到一个紧凑的探头中， 操作模式之间的无缝和快速切换。除了技术 开发和临床翻译，该项目的另一个目的是开发和测试针对性 提供更安全和更有效的卵巢癌体内鉴定的造影剂。

The objective of this research is to further develop and clinically validate a real-time multispectral confocal microendoscope for in vivo diagnosis of ovarian cancer. The confocal microendoscope is a new type of instrument for visualizing tissue at the cellular level and has shown great promise for performing optical biopsy. Confocal microendoscopy has the potential to provide a physician with an immediate evaluation of tissue and to survey a much greater area of tissue, reducing the sampling error of traditional tissue extraction biopsy. A confocal microlaparoscope system was constructed and tested in vivo in humans during the prior funding period. A specific aim of this work is to now validate the system and show that it can be used to detect cancer of the ovary during a laparoscopic procedure. Additional objectives of the work are to expand the use of the instrument to the detection of peritoneal implants of ovarian cancer throughout the abdominal cavity and to develop and test a system capable of imaging inside the fallopian tubes. Further technical development is aimed at improving the confocal imaging performance and adding optical coherence tomography as a complimentary imaging modality. The new instrument will incorporate the two imaging modalities into a single compact probe with seamless and rapid switching between modes of operation. In addition to the technology development and clinical translation, another aim of the project is to develop and test targeted contrast agents that provide safer and more effective in vivo identification of ovarian cancer.