Laboratory Core

CARC的分子/细胞生物学实验室核心在协调CARC研究组成部分和试点项目的研究活动方面发挥着基础性作用。此外，核心将扩展其专业知识，以支持NIAAA资助的与CARC主题相关的项目。它的使命将是作为进行实验程序、培训技术人员和开发尖端分析技术的核心资源，使我们的研究人员能够实现他们的CARC研究目标。分子/细胞生物学实验室核心的具体目标是：1.使用荧光激活细胞分选(FACS)设施进行细胞表型、功能分析和分选。2.根据CARC调查人员的需要进行分子生物学检测和程序。3.对中国食品药品监督管理局支持的项目进行特定的分析检测。4.维持细胞培养设施，并支持利用细胞培养技术的研究活动。除了四个具体目标外，CARC分子/细胞生物学实验室核心在扩大CARC调查人员可用的流式细胞术、基因克隆、Taqman分析、Luminex分析和ELISA方面的记录方面已有既定记录。核心的发展方面将侧重于CARC的目标，以确保它们的继承。其中包括：1)开发与FACS罕见细胞事件分类相关的新型Taqman聚合酶链式反应和RT-聚合酶链式反应分析方法；2)与LSUHSC的基因组学核心设施合作，扩展DNA甲基化和染色质乙酰化的分析方法，以调节特定基因的表达。将执行优先事项，以确保中心支持的研究项目的最大生产率。这一核心中的人员将培训其他人员，并将帮助协调项目之间的研究工作。分子/细胞生物学实验室核心的总体目标是提高研究参与者追求中心目标的效率、质量控制、合作和灵活性。

The CARC's Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the research activities of CARC research components and pilot projects. In addition, the Core will extend its expertise to support NIAAA funded projects that tie to the theme of the CARC. Its mission will be to serve as a core resource for conducting experimental procedures, training technical personnel, and developing cutting-edge analytical techniques that enable our investigators to achieve their CARC research objectives. The Specific Aims of the Molecular/Cellular Biology Laboratory Core are: 1. To perform cellular phenotyping, functional analysis, and sorting using fluorescent activated cell-sorting (FACS) facilities. 2. To perform molecular biological assays and procedures as needed by the CARC investigators. 3. To perform specific analytical assays for projects supported by the CARC. 4. To maintain a cell culture facility and support research activities utilizing cell culture technology. In addition to the four Specific Aims, the CARC Molecular/Cellular Biology Laboratory Core has an established track record of expanding the repertoire of flow cytometry, gene cloning, Taqman assays, luminex assays, and ELISAs available to CARC investigators. Developmental aspects of the Core will be focused on CARC objectives in order to ensure their succress. These include 1) developing novel Taqman PCR and RT-PCR assays linked to FACS sorting of rare cell events and 2) collaborating with the Geneomics Core Facility at LSUHSC to expand analytical protocols for determination of DNA methylation and chromatin acetylation in the regulation of specific gene expression. Priorities will be enforced to secure maximal productivity of research projects supported by the Center. The personnel in this core will train other personnel and will help coordinate research efforts among projects. The overall goal of the Molecular/Cellular Biology Laboratory Core is to enhance efficiency, quality control, cooperation, and flexibility of research participants in their pursuit of Center objectives.