SURFACE ENHANCED FLUORESCENCE NANOTAGS FOR ULTRASENSITIVE MULTIPLEXED ASSAY SYST

用于超灵敏多重检测系统的表面增强荧光纳米标记

• 批准号: 8341466
• 负责人: DAVID SEBBA
• 金额: $ 20万
• 依托单位: NANOCOMPOSIX, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2012-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8341466
• 关键词: Antibodies; Antigens; Binding; Biological Assay; Blinking; Cancer Detection; Cancer Diagnostics; Chemistry; Detection; Dot Immunoblotting; ERBB2 gene; Engineering; Enzyme-Linked Immunosorbent Assay; Excision; Exhibits; Fluorescence; Fluorescence Spectroscopy; Fluorescent Antibody Technique; Fluorescent Probes; Glutamate Carboxypeptidase II; Label; Link; Location; Malignant Neoplasms; Metals; Methods; Nanosphere; Nanotechnology; Optics; Performance; Quantum Dots; Sensitivity and Specificity; Signal Transduction; Silicon Dioxide; Silver; Solutions; Specificity; Surface; Survival Rate; TP53 gene; Testing; Thick; Time; Toxic effect; antibody engineering; base; fluorophore; improved; light emission; nanoengineering; nanoparticle; particle; plasmonics

Late detection is the primary cause of poor survival rates from cancer. Nanotechnology-enabled assays can detect early markers of cancer with much higher sensitivity than current detection methods. Existing assays use fluorescent probes with organic fluorophores or quantum dots, both of which have limitations. Organic fluorophores have limited brightness and photostability, reducing sensitivity and effective signal quantitation. Quantum dots exhibit toxicity and irregular blinking as well as broad emission spectra which reduces the level of multiplexing possible. We will develop a new class of ultrabright and ultrastable fluorescence probes, Surface Enhanced Fluorescence (SEF) Nanotags, to improve the sensitivity of fluorescence-based cancer detection. Nanoengineering of the SEF-nanotags to place fluorophores near the surface of plasmonic nanoparticles increases the light emission by up to a factor of 100X and significantly increases probe photostability. SEF probes will be functionalized with antibodies engineered to target cancer markers. The SEF nanotags will interrogate an analyte-containing solution and be detected on a microarray of complementary chemotropes. The brightness, photostability, and detection sensitivity of the SEF-nanotags will be compared to existing probes. These ultrabright and ultrastable SEF nanotags are easily adapted to existing fluorescence-based cancer diagnostics with improved sensitivity.

晚期发现是癌症存活率低的主要原因。纳米技术使能的测定可以检测癌症的早期标志物，其灵敏度比目前的检测方法高得多。现有的测定使用具有有机荧光团或量子点的荧光探针，这两者都具有局限性。有机荧光团具有有限的亮度和光稳定性，降低了灵敏度和有效的信号定量。量子点表现出毒性和不规则闪烁以及宽发射光谱，这降低了可能的多路复用水平。我们将开发一类新的超亮和超稳定的荧光探针，表面增强荧光（SEF）纳米标签，以提高基于荧光的癌症检测的灵敏度。将荧光团放置在等离子体纳米颗粒表面附近的SEF-nanotags的纳米工程使光发射增加高达100倍，并显着增加探针的光稳定性。SEF探针将用工程化以靶向癌症标志物的抗体功能化。SEF纳米标签将询问含有分析物的溶液，并在互补趋化因子的微阵列上检测。SEF纳米标签的亮度、光稳定性和检测灵敏度将与现有探针进行比较。这些超亮和超稳定的SEF纳米标签很容易适应现有的基于荧光的癌症诊断，具有更高的灵敏度。