DEVELOPMENT OF A BABOON MODEL OF LIVER CANCER

狒狒肝癌模型的研制

• 批准号: 8357717
• 负责人: Robert E LANFORD
• 金额: $ 3.56万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357717
• 关键词: Animal Model; Autologous; Cancer Etiology; Cell Transplants; Cells; Cessation of life; Development; Diagnosis; Diagnostic; Funding; Gene Transduction Agent; Gene-Modified; Genes; Genetic; Grant; Hepatocyte; Imaging Techniques; Lentivirus Vector; Liver; Malignant Neoplasms; Malignant neoplasm of liver; Methods; Modeling; Modification; National Center for Research Resources; Oncogenes; Papio; Primates; Principal Investigator; Property; Research; Research Infrastructure; Resources; SCID Mice; Source; Staging; System; United States National Institutes of Health; anticancer research; cancer therapy; cost; gene cloning; mouse model; new technology; programs; tumor; tumorigenic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is a pilot program to create a new model for liver cancer research and therapy. We will create a baboon model of liver cancer for use in new technologies involving advanced diagnostics and therapies using sophisticated imaging techniques to visualize and treat the tumor. Currently no large animal model of liver cancer is available to develop and perfect these methods. Liver cancer is the 4th leading cause of cancer death worldwide. Because it is a fast-growing tumor that is rarely diagnosed in the early stages, liver cancer is typically fatal within a year of diagnosis. In this program we will clone the genes involved in liver cancer from the baboon, such that all genes involved are homologous to the host. We will introduce genetic modifications to convert the genes into cancer inducing genes or oncogenes. We will use gene therapy vectors (Lentivirus vectors) to introduce multiple modified genes into liver cells (hepatocytes) growing in our well established hepatocyte culture system for baboon hepatocytes. Cells with tumorigenic properties will first be selected in the SCID mouse model and then introduced into the liver of baboon that was the original donor of the cells (autologous cell transplant). Once perfected, the model will be used to advance new therapies for liver cancer.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这是一个试点项目，旨在为肝癌研究和治疗创造一个新的模式。我们将创建一个狒狒肝癌模型，用于新技术，包括先进的诊断和治疗，使用复杂的成像技术来可视化和治疗肿瘤。目前还没有大型肝癌动物模型可用于开发和完善这些方法。肝癌是全球第四大癌症死亡原因。因为它是一种快速生长的肿瘤，很少在早期诊断出来，肝癌通常在诊断后一年内是致命的。在这个项目中，我们将从狒狒中克隆与肝癌相关的基因，这样所有相关的基因都与宿主同源。我们将引入基因修饰，将基因转化为癌症诱导基因或癌基因。我们将使用基因治疗载体（慢病毒载体）将多种修饰的基因导入在我们完善的狒狒肝细胞肝细胞培养系统中生长的肝细胞（肝细胞）中。首先在SCID小鼠模型中选择具有致瘤性的细胞，然后将其引入狒狒的肝脏中，狒狒是细胞的原始供体（自体细胞移植）。一旦完善，该模型将用于推进肝癌的新疗法。