GBV-B: A SMALL PRIMATE MODEL FOR HEPATITIS C INFECTION

GBV-B：丙型肝炎感染的小型灵长类动物模型

• 批准号: 8357644
• 负责人: Robert E LANFORD
• 金额: $ 5.71万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357644
• 关键词: Affect; Animal Model; Animals; Callithrix; Callithrix jacchus jacchus; Cirrhosis; Disease; Funding; GB virus B; Grant; Hepatitis; Hepatitis C; Hepatitis C virus; Hepatocyte; Infection; Liver diseases; Malignant neoplasm of liver; Modeling; National Center for Research Resources; Population; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Saguinus; Source; System; Testing; Time; United States National Institutes of Health; Virus; cost; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Hepatitis C virus infections affect approximately 2% of the worldwide population. The virus has a high propensity for inducing persistent infection that over time progress to serious liver disease including cirrhosis and liver cancer. Research on HCV has been impeded by the lack of a small animal model and a tissue culture system. GBV-B is the virus phylogenetically most closely related to HCV and it causes hepatitis in tamarins. We have developed the GBV-B animal model as a surrogate small primate model for HCV. We have recently demonstrated that the common marmoset is equally permissive for GBV-B infections and is now the preferred animal model. In addition, we have developed a robust tissue culture system for GBV-B utilizing primary hepatocyte cultures from tamarins or marmosets. In this proposal, we will more fully develop the animal and culture models for GBV-B and test specific hypotheses relevant to HCV disease.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 丙型肝炎病毒感染影响全球约 2% 的人口。 该病毒很容易引起持续感染，随着时间的推移，会发展为严重的肝脏疾病，包括肝硬化和肝癌。 由于缺乏小动物模型和组织培养系统，HCV 研究受到阻碍。 GBV-B 是系统发育上与 HCV 最密切相关的病毒，它会导致狨猴肝炎。 我们开发了 GBV-B 动物模型作为 HCV 的替代小灵长类动物模型。我们最近证明，普通狨猴同样容易感染 GBV-B，因此现在是首选的动物模型。 此外，我们利用狨猴或狨猴的原代肝细胞培养物，开发了一种强大的 GBV-B 组织培养系统。 在本提案中，我们将更全面地开发 GBV-B 的动物和培养模型，并测试与 HCV 疾病相关的具体假设。