NEXT GENERATION APPROACHES TO NON-HUMAN PRIMATE BIOINFORMATICS

非人类灵长类生物信息学的下一代方法

• 批准号: 8358004
• 负责人: Eric J. Vallender
• 金额: $ 1.38万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358004
• 关键词: Autistic Disorder; Bioinformatics; Brain; Coupling; DNA Resequencing; Data; Ensure; Environment; Funding; Genes; Genetic; Genome; Genomics; Grant; Growth; Human; Macaca mulatta; National Center for Research Resources; New England; Orthologous Gene; Phenotype; Primates; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Schizophrenia; Source; Technology; United States National Institutes of Health; Variant; base; cofactor; cost; exome; human disease; improved; new technology; next generation; nonhuman primate; tool; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. As we move into the post-genomic era new technologies have become available that allow for a greater and more complete understanding of non-human primate genetics. This project exploits these new opportunities to answer questions that have long existed and to improve existing studies. Specifically, we are using xenohybridization approaches to capture the complete exomes of rhesus macaques as well as other non-human primates prior to complete resequencing using next generation technologies. We will then use this information to improve the existing genomes, including identifying mistakes, fill in gaps, and identify variation. This will also allow us to better characterize the evolutionary changes at the genomic level, including better annotation of non-human primate orthologs and characterization of evolutionary rates of change across species and genes. We also are using this information to compare evolutionary rates of change for groups of genes associated with specific human diseases; schizophrenia and autism in particular have been hypothesized to have been derived from human-specific changes to the brain. Coupling with this growth in the quantity and quality of sequence information is the need for a robust bioinformatic infrastructure for accessing and integrating the data into ongoing research. Here we are developing tools that allow for a rapid and complete incorporation of genetic information into existing phenotypes, ensuring that researchers can immediately include genetics as a cofactor in their non-human primate research and to explore the relationship between genetics and environment in emerging traits.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 随着我们进入后基因组时代，新技术已经出现，可以更好地了解非人类灵长类动物的遗传学。该项目利用这些新的机会来回答长期存在的问题，并改进现有的研究。具体来说，我们正在使用异种杂交方法来捕获恒河猴以及其他非人灵长类动物的完整外显子组，然后使用下一代技术完成重新测序。然后，我们将使用这些信息来改进现有的基因组，包括识别错误，填补空白和识别变异。这也将使我们能够更好地表征基因组水平上的进化变化，包括更好地注释非人类灵长类动物的直系同源物，以及表征物种和基因之间的进化变化率。我们还利用这些信息来比较与特定人类疾病相关的基因组的进化变化率;特别是精神分裂症和自闭症，已经被假设为源自人类特定的大脑变化。随着序列信息数量和质量的增长，需要一个强大的生物信息学基础设施来访问数据并将其整合到正在进行的研究中。在这里，我们正在开发工具，允许快速和完整地将遗传信息整合到现有的表型中，确保研究人员可以立即将遗传学作为辅助因素纳入他们的非人类灵长类动物研究，并探索遗传学和环境之间的关系。