COBRE: UNE MED CTR: CORE A: ADMINISTRATIVE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 神经感觉功能的丧失，特别是听觉和视觉的丧失，会对沟通、教育、职业和生活质量造成毁灭性的影响。由于内耳和视网膜的神经感觉细胞在受损或退化后不会被替换，因此这种损失是永久性的。内布拉斯加州神经感觉系统分子生物学中心的目的是描述控制神经感觉功能发育和维持的遗传机制以及与相关基因突变相关的相应病理，以确定潜在的干预途径。我们将专注于有前景的再生医学策略，如通过使用多能干细胞重建受损细胞，或内耳和视网膜中有丝分裂后细胞的去分化和再生。这些研究利用专门的动物模型，允许在发育的特定关键点控制基因表达，并在分子和表型水平上对相关发现进行详细评估。为了促进这些研究，3个独立研究机构联合建立了一个互动中心，采用多学科方法，旨在实现这些明确的目标。我们拥有3名高级研究人员和一个模范的外部咨询委员会，成功地指导了14名初级教员，并创建了一个通过类似的主题和机械方法团结在一起的协作小组。我们的研究得到了三个科学核心的推动：一个用于发展小鼠模型的小鼠基因组工程核心，一个用于全面评估基因表达水平和确定可能的遗传网络的微阵列核心，以及一个用于研究实验改变结果的分子表型/组织学核心。这些核心是对所有三个机构研究基础设施的重要补充。在这个Cobre项目的第二阶段，我们将继续支持6名初级研究人员：2名正在研究失明和聋盲/失明的特定症状的机制，1名专注于视网膜干细胞群体的发展和丰富，3名正在研究内耳分化的分子途径，目的是促进感觉再生。随着这些项目获得外部资金和中心资金的轮换，将增加新的项目。新的调查人员的加入，加上对研究活动和核心设施运行的持续支持，将确保不断扩大的合作“临界量”，使中心得以维持下去，并使参与者能够获得确保其长期生存的外部资金。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The loss of neurosensory function, particularly the sensations of hearing and vision, has devastating effects on communication, education, occupation, and quality of life. Since neurosensory cells of the inner ear and retina are not replaced after damage or degeneration, such losses are permanent. The purpose of the Nebraska Center for the Molecular Biology of Neurosensory Systems is to characterize the genetic mechanisms controlling the development and maintenance of neurosensory functions and the corresponding pathology associated with relevant gene mutations to identify potential avenues for intervention. We will focus our efforts on promising regenerative medicine strategies such as the reconstitution of damaged cells through the use of pluripotent stem cells or de-differentiation and regeneration of post-mitotic cells in the inner ear and retina. These studies utilize specialized animal models that allow control of gene expression at specific critical points in development, with detailed evaluation of the associated findings at the molecular and phenotypic levels. To facilitate these studies, 3 independent research institutions have joined to build an interactive Center with a multidisciplinary approach designed to address to these well-defined objectives. With a core of 3 senior researchers and an exemplary External Advisory Committee, we have successfully mentored 14 junior faculty members and created a collaborative group united by similar thematic and mechanistic approaches. Our research has been facilitated by 3 scientific cores: a Mouse Genome Engineering core for the development of mouse models, a Microarray core for comprehensive assessment of gene expression levels and determination of possible genetic networks, and a Molecular Phenotyping/Histology core to study the results of experimental alterations. These cores are an important addition to the research infrastructure of all 3 institutions. In the second phase of this COBRE project, we will continue to support 6 junior researchers: 2 are studying the mechanisms of specific syndromes of blindness and deaf/blindness, 1 is concentrating on the development and enrichment of retinal stem cell populations, and 3 are studying the molecular pathways of inner ear differentiation with the goal of facilitating sensory regeneration. As those projects obtain external funding and rotate off Center funding, new projects will be added. The addition of new investigators, along with continued support of research activities and the operation of core facilities, will ensure a growing "critical mass" of collaboration that will sustain the Center, and enable participants to obtain external funding which will ensure its long term viability.