CONFOCAL MICROFLUOROMETRY & MICROSCOPY CORE

共焦显微荧光测定

• 批准号: 8360333
• 负责人: A. Philine Wangemann
• 金额: $ 26.51万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360333
• 关键词: Centers of Research Excellence; Color; Cytophotometry; Disease; Epithelial; Fingerprint; Funding; Grant; Health; Imagery; Mentors; Microscopy; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Scanning; Signal Transduction; Source; Techniques; Tissues; Training; United States National Institutes of Health; cost; instrumentation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The COBRE Confocal Microfluorometry and Microscopy Core provides instrumentation, expertise, training and mentoring in confocal microfluorometry and microscopy. Capabilities include: microfluorometry of fast Ca2+ signals in cultured and native epithelial tissues, subcellular visualization of molecules, co-localization experiments where precise resolution and color separation through emission fingerprinting are necessary, and advanced co-localization techniques where emission scanning is critical.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Cobre共焦显微荧光和显微镜核心提供共聚焦显微荧光和显微镜方面的仪器、专业知识、培训和指导。其功能包括：培养的和自然的上皮组织中快速钙信号的显微荧光测量，分子的亚细胞可视化，需要通过发射指纹进行精确分辨率和颜色分离的共定位实验，以及发射扫描至关重要的高级共定位技术。