CONFOCAL MICROFLUOROMETRY & MICROSCOPY CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。Cobre共焦显微荧光测量和显微镜核心设施提供推动所有Cobre项目所需的共聚焦显微荧光测量和显微镜方面的仪器、专业知识、培训和指导。这些项目严重依赖于：培养的和本地的上皮组织中快速钙信号的显微荧光测量；分子的亚细胞可视化，包括标记的三维重建；需要通过发射指纹进行最大分辨率和颜色分离的共定位实验；以及先进的共定位技术，例如FRET，我们设备的发射扫描能力对此至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE Confocal Microfluorometry and Microscopy Core facility provides the instrumentation, expertise, training and mentoring in confocal microfluorometry and microscopy necessary for the advancement of all COBRE projects. The projects are critically dependent on: microfluorometry of fast Calcium signals in cultured and native epithelial tissues; subcellular visualization of molecules, including the 3-dimensional reconstruction of labeling; colocalization experiments where maximal resolution and color separation through emission fingerprinting are necessary; and advanced colocalization techniques, such as FRET, for which the emission-scanning capabilities of our equipment is critical.