CONFOCAL MICROFLUOROMETRY & MICROSCOPY CORE

共焦显微荧光测定

• 批准号: 7381858
• 负责人: A. Philine Wangemann
• 金额: $ 18.36万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381858
• 关键词: CONFOCAL; MICROFLUOROMETRY; MICROSCOPY; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE Confocal Microfluorometry and Microscopy Core facility provides the instrumentation, expertise, training and mentoring in confocal microfluorometry and microscopy necessary for the advancement of all COBRE projects. The projects are critically dependent on: microfluorometry of fast Calcium signals in cultured and native epithelial tissues; subcellular visualization of molecules, including the 3-dimensional reconstruction of labeling; colocalization experiments where maximal resolution and color separation through emission fingerprinting are necessary; and advanced colocalization techniques, such as FRET, for which the emission-scanning capabilities of our equipment is critical.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。COBRE共焦显微荧光测量和显微镜核心设施提供了所有COBRE项目进步所需的共焦显微荧光测量和显微镜的仪器，专业知识，培训和指导。这些项目主要依赖于：培养和天然上皮组织中快速钙信号的显微荧光测定;分子的亚细胞可视化，包括标记的三维重建;共定位实验，其中需要通过发射指纹进行最大分辨率和颜色分离;以及先进的共定位技术，如FRET，我们设备的发射扫描能力至关重要。