CONFOCAL MICROFLUOROMETRY & MICROSCOPY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Cobre共焦显微荧光测量和显微镜核心设施提供推动所有Cobre项目所需的共聚焦显微荧光测量和显微镜方面的仪器、专业知识、培训和指导。这些项目严重依赖于：培养的和本地的上皮组织中快速钙信号的显微荧光测量；分子的亚细胞可视化，包括标记的三维重建；需要通过发射指纹进行最大分辨率和颜色分离的共定位实验；以及先进的共定位技术，例如FRET，我们设备的发射扫描能力对此至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE Confocal Microfluorometry and Microscopy Core facility provides the instrumentation, expertise, training and mentoring in confocal microfluorometry and microscopy necessary for the advancement of all COBRE projects. The projects are critically dependent on: microfluorometry of fast calcium signals in cultured and native epithelial tissues; subcellular visualization of molecules, including the 3-dimensional reconstruction of labeling; colocalization experiments where maximal resolution and color separation through emission fingerprinting are necessary; and advanced colocalization techniques, such as FRET, for which the emission-scanning capabilities of our equipment is critical.