MOLECULAR MECHANISMS OF MRNA STABILITY IN HUMAN SALIVA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 mRNA的稳定和不稳定在基因表达调控中起着重要作用。 我们对人类mRNA的存在以及使其能够存在于人类唾液中的稳定性的了解非常少。唾液中病毒、细菌和人类RNA的存在使我们能够研究各种疾病的mRNA稳定性的机制和调控。 与人类唾液中mRNA稳定性的调节有关的挑战，以及如何通过利用mRNA稳定性途径来稳定mRNA尚未发现。我们假设，有序列元件和蛋白质因素负责唾液或口腔癌患者的疾病特异性RNA生物标志物的差异存在。这项研究将使我们能够深入了解负责人类唾液中mRNA稳定性的序列元件和蛋白质因子。因此，该提案的主要目标是了解人类唾液中mRNA稳定性的机制方面。为实现这些目标，提出了两个具体目标。I）确定负责人唾液中mRNA稳定化的基本机制，和II）研究与MAP激酶途径活化相关的含有富含AU的元件的mRNA衰变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Stabilization and destabilization of mRNA plays an important role in the regulation of gene expression. Our knowledge of presence of human mRNA and the stabilization that enables its presence in human saliva is very poor. The existence of viral, bacterial and human RNAs in saliva allows us to study the mechanisms and regulations of mRNA stability for various diseases. The challenges related to regulation of mRNA stability in human saliva, and how mRNA is stabilized by utilizing mRNA stability pathways is yet to uncover. We hypothesize that there are sequence elements and protein factors responsible for the differential presence of disease specific RNA biomarkers in saliva or oral cancer patients. This research will allow us to gain insights into the sequence elements and protein factors responsible for mRNA stability in human saliva. Therefore, the major goal of this proposal is to understand the mechanistic aspects of mRNA stability in human saliva. Two specific aims are proposed to accomplish these goals. I) Determine the fundamental mechanisms responsible for the stabilization of mRNA's in human saliva and II) Validate the AU-rich element containing mRNA decay in correlation with MAP kinase pathway activation.