MOLECULAR MECHANISMS OF MRNA STABILITY IN HUMAN SALIVA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 MRNA的稳定和失稳在基因表达的调控中起着重要的作用。我们对人类mRNA的存在以及使其在人类唾液中存在的稳定性的了解非常有限。唾液中病毒、细菌和人类RNA的存在使我们能够研究各种疾病的mRNA稳定的机制和调节。与调节人类唾液中的mRNA稳定性有关的挑战，以及如何利用mRNA稳定途径来稳定mRNA的问题尚未被揭开。我们假设，在唾液或口腔癌患者中，存在导致疾病特异性RNA生物标记物差异存在的序列元件和蛋白质因素。这项研究将使我们能够深入了解导致人类唾液中mRNA稳定的序列元件和蛋白质因素。因此，这项建议的主要目标是了解人类唾液中信使核糖核酸稳定性的机制。为实现这些目标，提出了两个具体目标。1)确定人唾液中mRNA稳定的基本机制；2)验证富含AU元件的mRNA衰变与MAP激酶途径激活的相关性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Stabilization and destabilization of mRNA plays an important role in the regulation of gene expression. Our knowledge of presence of human mRNA and the stabilization that enables its presence in human saliva is very poor. The existence of viral, bacterial and human RNAs in saliva allows us to study the mechanisms and regulations of mRNA stability for various diseases. The challenges related to regulation of mRNA stability in human saliva, and how mRNA is stabilized by utilizing mRNA stability pathways is yet to uncover. We hypothesize that there are sequence elements and protein factors responsible for the differential presence of disease specific RNA biomarkers in saliva or oral cancer patients. This research will allow us to gain insights into the sequence elements and protein factors responsible for mRNA stability in human saliva. Therefore, the major goal of this proposal is to understand the mechanistic aspects of mRNA stability in human saliva. Two specific aims are proposed to accomplish these goals. I) Determine the fundamental mechanisms responsible for the stabilization of mRNA's in human saliva and II) Validate the AU-rich element containing mRNA decay in correlation with MAP kinase pathway activation.