MICROBIAL METABOLIC MUTUALISM UNDER EXTREME STARVATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我的项目的目标是了解在极端饥饿条件下支持微生物生长的代谢活动。在理解在饥饿环境中维持微生物群落的相互作用和过程中，我们希望更好地理解微生物生态学的一个突出问题：为什么在环境中发现的大多数生物在实验室是不可培养的？这种不可培育性被认为是由于无法复制这些物种中许多挑剔的生长要求，使它们在环境中可以生存，但在实验室条件下无法培养。通过这一提议，我们的目标是检验我们的假设，即微生物群落通过建立相互作用，使物种能够更有效地利用进入系统的复杂营养源，在极端营养稀少的环境中生存。虽然这种相互作用可能会促进极端饥饿下的多样性，但专性的互惠关系阻止了使用传统技术培育这些物种。拟议的实验旨在了解进入这些系统的可用能量的数量和类型对群落多样性和互惠共生的作用。其具体目标是：1)确定微生物在极端饥饿条件下是否发生互惠相互作用，以及它们在支持群落生长方面发挥什么作用；2)确定这种互惠作用是否增加了极端饥饿条件下的可用能源和代谢效率，从而推动群落多样性；以及3)通过分离以前相互依赖的物种，增加这些极端贫瘠环境中以前无法培养的细菌物种的数量。通过使用多种技术组合来监测营养物质流动如何影响群落多样性，我们努力了解一些更复杂的代谢相互作用，这些作用能够支持饥饿下的生命，同时增加可培养微生物物种的多样性和数量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of my project is to understand the metabolic activity that supports microbial growth under extreme starvation. In understanding the interactions and processes that sustain microbial communities in starved environments, we hope to better understand one of the outstanding questions of microbial ecology: why are the majority of organisms identified in the environment unculturable in the laboratory? This unculturability is presumed to be due to the inability to reproduce the fastidious growth requirements of many of these species, making them viable within the environment, but unculturable under laboratory conditions. Through this proposal, we aim to test our hypothesis that microbial communities subsist in extremely oligotrophic environments by establishing mutualistic interactions, allowing species to more efficiently utilize the complex nutrient sources entering the system. While such interactions may promote diversity under extreme starvation, obligate mutualism prevents cultivation of these species using traditional techniques. The experiments proposed are geared toward understanding the role that the amount and type of available energy entering these systems have on community diversity and mutualism. The specific aims are: 1) to determine whether microbial mutualistic interactions are occurring under extreme starvation conditions and what role they play in supporting community growth; 2) to determine whether such mutualism increases the usable energy sources and metabolic efficiency under extreme starvation, driving community diversity; and 3) to increase the number of previously unculturable bacterial species from these extremely oligotrophic environments by separating previously interdependent species. By using a combination of techniques to monitor how nutrient flow affects community diversity, we endeavor to understand some of the more complex metabolic interactions capable of supporting life under starvation, while increasing the diversity and number of culturable microbial species.