GENETIC & METABOLIC ADAPTATION BY SALMONELLA TO THE NATURAL ENVIRONMENT

基因

• 批准号: 7610385
• 负责人: HAZEL A BARTON
• 金额: $ 13.16万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610385
• 关键词: Cobalamin; Computer Retrieval of Information on Scientific Projects Database; Condition; Cytoplasmic Granules; Environment; Escherichia coli; Ethanolamines; Frequencies; Funding; Genes; Genetic; Grant; Institution; Life Style; Metabolic; Minerals; Organism; Pathogenesis; Process; Propylene Glycols; Relative (related person); Research; Research Personnel; Resources; Respiration; Salmonella; Soil; Source; Sulfides; Sulfites; Sulfur; Sulfur Compounds; Thinking; United States National Institutes of Health; Work; ethanolamine; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Salmonella is generally considered a low-frequency commensal and low-grade pathogen with very limited ability to survive long term in the environment outside of a host organism. Recent work has identified a constellation of interactive metabolic functions shared by all Salmonellae, but absent from its nearest relative E. coli. The constellation of Salmonella-specific functions includes de novo synthesis of cobalamin (B12), use of B12 in degradation of ethanolamine and propanediol, anaerobic respiration of tetrathionate to thiosulfate (ttr genes), and subsequent reduction to sulfite (phs genes) and sulfide (asr genes). All these functions would be used when Salmonella grows anaerobically on ethanolamine or propanediol in the presence of reducible sulfur compounds. Under these conditions, Salmonella forms granules of mineral sulfur, which it (but not E. coli) can later reduce by a process that requires the phs genes. While these functions may be important to pathogenesis, we think it more likely that they suggest a significant lifestyle for Salmonella in particular soil environments. Our work has been geared to understanding whether there is an environmental niche for Salmonella and the potential impact that such an ecological niche will have on our understanding of the pathogenesis of this organism.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 沙门氏菌通常被认为是一种低频率的寄生虫和低级病原体，在宿主生物体外的环境中长期存活的能力非常有限。最近的工作已经确定了所有鲑科鱼类共有的一系列相互作用的代谢功能，但其最近的亲戚E。杆菌 沙门氏菌特异性功能的集合包括钴胺素（B12）的从头合成、在乙醇胺和丙二醇的降解中使用B12、连四硫酸盐到硫代硫酸盐的厌氧呼吸（ttr基因）以及随后还原成亚硫酸盐（phs基因）和硫化物（asr基因）。 当沙门氏菌在乙醇胺或丙二醇上厌氧生长时，在可还原的硫化合物的存在下，所有这些功能都将被使用。 在这些条件下，沙门氏菌形成矿物质硫颗粒，它（但不是E。大肠杆菌）可以随后通过需要phs基因的过程来还原。 虽然这些功能可能对发病机制很重要，但我们认为它们更可能表明沙门氏菌在特定土壤环境中的重要生活方式。 我们的工作是为了了解沙门氏菌是否存在一个环境生态位，以及这种生态位对我们理解这种生物的发病机制的潜在影响。