T-TUBULES IN MLP KNOCK OUT MOUSE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本研究旨在阐明心力衰竭相关的包括横管在内的心肌膜细胞器的异常。研究包括对从人类扩张型心肌病突变小鼠模型获得的心肌进行EM和LM分析。NCMIR开发的记录T管三维结构的独特组合技术有望揭示其解剖特征，其生物学意义远远超过迄今已知的该膜结构域的性质，这对于理解生理-病理心脏兴奋-收缩耦合至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This study aims to elucidate the heart failure-assoicated abnormalities in cardiac membrane organelles including transverse tubules (T-tubules). Studies include the EM and LM analyses of myocardium obtained from a mutant mouse model of human dilated cardiomyopathy. The unique combinatory technology developed by NCMIR to document 3D structure of T-tubes are expected to unveil its anatomical features with biological implications far greater than the hitherto recognized nature of this membrane domain, which is critical to understand the physio-pathology cardiac excitation-contraction coupling.