T-TUBULES IN MLP KNOCK OUT MOUSE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本研究旨在阐明心力衰竭相关的心脏膜细胞器，包括横小管（T-小管）的异常。 研究包括从人扩张型心肌病突变小鼠模型获得的心肌的EM和LM分析。 NCMIR开发的独特组合技术记录了T管的3D结构，预计将揭示其解剖学特征，其生物学意义远远大于迄今为止公认的这种膜结构域的性质，这对于理解生理病理学心脏兴奋-收缩耦合至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This study aims to elucidate the heart failure-assoicated abnormalities in cardiac membrane organelles including transverse tubules (T-tubules). Studies include the EM and LM analyses of myocardium obtained from a mutant mouse model of human dilated cardiomyopathy. The unique combinatory technology developed by NCMIR to document 3D structure of T-tubes are expected to unveil its anatomical features with biological implications far greater than the hitherto recognized nature of this membrane domain, which is critical to understand the physio-pathology cardiac excitation-contraction coupling.