MULTISCALE CHARACTERIZATION OF VIRAL VECTOR-INDUCED GLIOBLASTOMAS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本项目的目的是在多形性胶质母细胞瘤(GBM)小鼠模型中研究伴随肿瘤形成的分子事件。一些感兴趣的目标包括血管生成标记物、细胞增殖指示物和细胞特异性标记物，以跟踪特定细胞群体的区域变化。我们将使用NCMIR首创和改进的技术组合来研究这些靶点在GBM进展过程中的分布和动态行为，包括大规模测绘和相关的光学和电子显微镜成像，以跟踪神经胶质细胞的精细过程以及这些细胞在受影响的脑区增殖时的相关细胞和分子相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to investigate the molecular events accompanying formation of tumors in a mouse model of glioblastoma multiforme (GBM). Some targets of interest include angiogenic markers, indicators of cell proliferation, and cell specific markers to track regional changes in specific cell populations. We will investigate the distribution and dynamic behavior of these targets during GBM progression using a combination of techniques being pioneered and refined at NCMIR including large-scale mapping and correlated light and electron microscopic imaging to follow the fine processes of glial cells and associated cellular and molecular interactions as these cells proliferate in the affected brain regions.