INVESTIGATIONS OF ALPHA-SYNUCLEIN IN PARKINSONIAN PATHOGENESIS

α-突触核蛋白在帕金森病发病机制中的研究

• 批准号: 8361914
• 负责人: JULIA B GEORGE
• 金额: $ 3.7万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361914
• 关键词: Collaborations; Electrons; Funding; Goals; Grant; Hydroxybutyrates; Image; Image Analysis; Investigation; Label; Life; Microscopic; National Center for Research Resources; Oxidoreductase; Parkinson Disease; Parkinsonian Disorders; Pathogenesis; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Role; Source; System; Techniques; Therapeutic; United States National Institutes of Health; Work; alpha synuclein; cost; improved; protein transport; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goals in this collaboration are to investigate the role of alpha-synuclein and hydroxybutyrate dehydrogenase type 2 (BDH2) in the progression of Parkinson disease (PD) and assess putative therapeutic strategies. We intend to apply the tetracysteine (4Cys)-labeling system developed at NCMIR to track, at multiple resolution scales, the trafficking of proteins associated with PD. This work will draw upon the techniques being advanced at NCMIR, including improved 4Cys approaches, correlated live imaging-3D electron microscopic analysis, and high-resolution electron tomographic reconstruction.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们在这项合作中的目标是研究α-突触核蛋白和羟基丁酸脱氢酶2型（BDH 2）在帕金森病（PD）进展中的作用，并评估推定的治疗策略。我们打算应用NCMR开发的四半胱氨酸（4Cys）标记系统，以多分辨率尺度跟踪与PD相关的蛋白质的运输。这项工作将借鉴先进的技术在NCMIR，包括改进的4Cys方法，相关的实时成像-三维电子显微镜分析，和高分辨率电子断层扫描重建。