INVESTIGATIONS OF ALPHA-SYNUCLEIN IN PARKINSONIAN PATHOGENESIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 我们在这项合作中的目标是研究α-突触核蛋白和羟基丁酸脱氢酶2型（BDH 2）在帕金森病（PD）进展中的作用，并评估推定的治疗策略。我们打算应用NCMR开发的四半胱氨酸（4Cys）标记系统，以多分辨率尺度跟踪与PD相关的蛋白质的运输。这项工作将借鉴先进的技术在NCMIR，包括改进的4Cys方法，相关的实时成像-三维电子显微镜分析，和高分辨率电子断层扫描重建。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goals in this collaboration are to investigate the role of alpha-synuclein and hydroxybutyrate dehydrogenase type 2 (BDH2) in the progression of Parkinson disease (PD) and assess putative therapeutic strategies. We intend to apply the tetracysteine (4Cys)-labeling system developed at NCMIR to track, at multiple resolution scales, the trafficking of proteins associated with PD. This work will draw upon the techniques being advanced at NCMIR, including improved 4Cys approaches, correlated live imaging-3D electron microscopic analysis, and high-resolution electron tomographic reconstruction.