CELL WALL ANALYSIS OF BRACHYPODIUM TRANSGENES

短柄草转基因的细胞壁分析

• 批准号: 8361258
• 负责人: JOHN RALPH
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361258
• 关键词: Cell Wall; Collaborations; Dimethyl Sulfoxide; Funding; Grant; Mutation; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Source; Transgenes; United States National Institutes of Health; cost; improved; pyridine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Brachypodium samples have been grown with CAD and PAL deficient transgene lines to improve their digestibility. These were be ball milled and prepared in DMSO-pyridine. 1H-13C HSQC spectra will be taken to examine the effects of this mutation on the cell wall of the samples. Analysis will be done chemometrically in collaboration with the Ralph lab to determine any relevant changes in the cell wall between samples.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 腕第一样品已用CAD和PAL不足的转基因线生长，以提高其消化率。这些是磨球并在DMSO吡啶中制备的。 1H-13C HSQC光谱将用于检查该突变对样品细胞壁的影响。分析将与Ralph Lab合作进行化学计量，以确定样品之间细胞壁的任何相关变化。