VESOSOME

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Vesosome是一种由载药脂质体包裹在另一个双层中的新型多室结构，与单层脂质体相比具有更好的保留率和稳定性，是一种很有前途的药物载体。目前的泡囊小体制剂利用饱和脂类的交指相，使脂双层在低温下形成平坦、开放的片层，在较高温度下接近形成大的单层囊泡。在闭合过程中，交错的薄片包裹其他脂泡或胶体颗粒，成为囊泡的外膜。结果表明，通过将三嵌段共聚物添加到由二棕榈醇-磷脂酰胆碱制成的交错指状薄片中，囊泡体达到了适合临床使用的大小。同时，吸收波长在可见光和近红外(NIR)范围内的中空金纳米球是引发药物载体内容物释放的良好候选者。低温电子显微镜证实，囊泡内含有中空的金纳米球。此外，还将6-羧基荧光素与金球包裹在一起，研究了飞秒激光脉冲照射下的囊泡释放情况。仅在少数激光脉冲作用下，就释放了大量的羧基荧光素。我们的结果表明，含有中空金纳米球的囊泡具有很大的载药量，可用于激光诱导治疗药物的输送和生物系统中激光的其他应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Vesosome, a new multi-compartment structure consisting of drug-loaded liposomes encapsulated within another bilayer, is a promising drug carrier with better retention and stability compared with unilamellar liposomes. The current vesosome preparation takes advantage of the interdigitated phase of saturated lipids, which causes lipid bilayers to form flat, open sheets at low temperature, that close to form large unilamellar vesicles at higher temperatures. During this closure, the interdigitated sheets encapsulate other lipid vesicles or colloidal particles to become the outer membrane of the vesosome. It has been shown that by adding the triblock copolymer to the interdigitated sheets made from dipalmitol-phosphatidylcholine, the vesosomes achieve the proper size for clinical use. Meanwhile, hollow gold nanospheres with absorbance wavelength in the visible and near infrared (NIR) range are good candidates to trigger the release of contents from drug carriers. Vesosomes containing hollow gold nanospheres in their aqueous interior compartments have been verified by cryo electron transmission microscopy. Furthermore, 6-carboxyfluorescein has been encapsulated together with gold spheres to investigate the release from vesosomes irradiated with femetosecond laser pulses. Substantial amounts of carboxyfluorescein were released in response to only a few laser pulses. Our results suggest vesosomes containing hollow gold nanospheres, with large loading capacity, are useful for laser induced delivery of therapeutic agents and other applications of lasers in biological systems.