BROME MOSAIC VIRUS

雀麦花叶病毒

• 批准号: 8361095
• 负责人: Cheng C Kao
• 金额: $ 2.45万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361095
• 关键词: Arginine; Biological; Brome mosaic virus; Capsid; Charge; Collaborations; Electron Microscopy; Funding; Goals; Grant; Image; Mutation; N-terminal; National Center for Research Resources; Principal Investigator; Property; RNA; Research; Research Infrastructure; Resources; Roentgen Rays; Source; Structure; United States National Institutes of Health; Viral; Virion; alpha helix; arm; cost; interest; macromolecule; mutant; particle; protein structure; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have made a mutant Brome mosaic virus (BMV) that has an interesting biological property. The mutations increase the positive charges inside the viral particles through the addition of two alpha-helices in the N-terminal arginine-rich arm in the capsid subunit of BMV. The X-ray crystal structure of the protein shell of BMV has been solved, but there is no information on the inner layer of RNA and how the N-terminal arm interacts with the RNA. We propose a collaboration where we can use cryo-EM to image and reconstruct the wild-type and mutant BMV. We will then trace the N-terminal arm of the two particles and identify how it interacts with RNA. The goal of this project is to visualize this N-terminal arm that is critical for interaction with the packaged viral RNAs and the structure of the RNAs inside the virion.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们已经制造了具有有趣生物学特性的突变体镶嵌病毒（BMV）。突变通过在BMV的衣壳亚基中添加了两个富含N端精氨酸的臂中的两个α-螺旋，从而增加了病毒颗粒内部的正电荷。 BMV蛋白壳的X射线晶体结构已得到解决，但是RNA的内层没有信息以及N末端臂如何与RNA相互作用。 我们提出了一项合作，可以在其中使用Cryo-EM来形象和重建野生型和突变体BMV。 然后，我们将追踪两个颗粒的N末端组，并确定其与RNA相互作用的方式。 该项目的目的是可视化与与包装病毒RNA相互作用和病毒体内RNA结构至关重要的N端臂。