MOUSE ROD OUTER SEGMENT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 光传导是一种信号级联反应，光子吸收被转化为电流的变化。光转导级联的成员大量存在于杆状感光器的细小的外节隔室中。这些蛋白质中的许多已经结晶，并推导出高分辨率结构。然而，目前还不知道这些蛋白质是如何组装的。 这项建议的目的是为了详细了解脊椎动物杆状感光器中的信号复合体。陈的实验室已经制造了一些定向缺失这些蛋白的转基因小鼠。对这些小鼠的外部节段进行断层扫描可能有助于重建参与光转导的蛋白质复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Phototransduction is a signaling cascade whereby photon absorption is converted into a change in electrical current. Members of the phototransduction cascade are abundantly present in the thin outer segment compartment of the rod photoreceptor. Many of these proteins have been crystallized and high resolution structures have been deduced. However, it is not known how these proteins are assembled. The objective of this proposal is to obtain a detailed understanding of the signaling complexes in the vertebrate rod photoreceptor. The Chen laboratory has made a number of transgenic mice with targeted deletion of these proteins. Tomography of outer segments from these mice may be helpful in the reconstruction of the protein complexes involved in phototransduction.