CHRISTOPHER GARCIA PRT TIME

克里斯托弗·加西亚 PRT 时间

• 批准号: 8362043
• 负责人: K CHRISTOPHER R GARCIA
• 金额: $ 0.27万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362043
• 关键词: Cell Surface Receptors; Complex; Crystallization; Data; Disease; Extracellular Domain; Funding; Grant; Health; Housing; Human; Immune system; Laboratories; Ligands; Measures; National Center for Research Resources; Nervous system structure; Principal Investigator; Radiation; Recombinant Proteins; Research; Research Infrastructure; Resources; Source; Synchrotrons; System; United States National Institutes of Health; cost; cross reactivity; interest; molecular recognition; protein complex; receptor; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. My laboratory is interested in structural aspects of cell-surface receptor/ligand interactions with relevance to human health and disease. We are engaged in expression and crystallization studies of numerous receptor extracellular domains both alone and in complex with their ligands. The receptor systems we study are primarily in the immune and nervous systems, with a special emphasis on understanding cross-reactivity in molecular recognition. Most of our projects are critically dependent on the ability to collect x-ray data at a synchrotron facility due to the generally small and moderate quality of the crystals which we can grow of large and heterogeneous protein complexes. In-house x-ray sources are not sufficient to measure useful data on most of our crystals, which are often few in number and extemely labor intensive to produce from the mammalian expression sources we utilize to make recombinant proteins.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我的实验室对与人类健康和疾病相关的细胞-表面受体/配体相互作用的结构方面感兴趣。我们致力于许多受体胞外结构域的表达和结晶研究，既有单独的，也有与其配体形成的复合体。我们研究的受体系统主要是在免疫和神经系统中，特别强调了解分子识别中的交叉反应。我们的大多数项目都严重依赖于在同步加速器设施中收集X射线数据的能力，因为我们可以生长的大型和异质蛋白质复合体的晶体通常很小和中等质量。内部的X射线源不足以测量我们大多数晶体上的有用数据，这些晶体的数量往往很少，而且从我们用来制造重组蛋白质的哺乳动物表达来源中生产出来的劳动密集型。