CHRISTOPHER GARCIA PRT TIME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我的实验室对与人类健康和疾病相关的细胞-表面受体/配体相互作用的结构方面感兴趣。我们致力于许多受体胞外结构域的表达和结晶研究，既有单独的，也有与其配体形成的复合体。我们研究的受体系统主要是在免疫和神经系统中，特别强调了解分子识别中的交叉反应。我们的大多数项目都严重依赖于在同步加速器设施中收集X射线数据的能力，因为我们可以生长的大型和异质蛋白质复合体的晶体通常很小和中等质量。内部的X射线源不足以测量我们大多数晶体上的有用数据，这些晶体的数量往往很少，而且从我们用来制造重组蛋白质的哺乳动物表达来源中生产出来的劳动密集型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. My laboratory is interested in structural aspects of cell-surface receptor/ligand interactions with relevance to human health and disease. We are engaged in expression and crystallization studies of numerous receptor extracellular domains both alone and in complex with their ligands. The receptor systems we study are primarily in the immune and nervous systems, with a special emphasis on understanding cross-reactivity in molecular recognition. Most of our projects are critically dependent on the ability to collect x-ray data at a synchrotron facility due to the generally small and moderate quality of the crystals which we can grow of large and heterogeneous protein complexes. In-house x-ray sources are not sufficient to measure useful data on most of our crystals, which are often few in number and extemely labor intensive to produce from the mammalian expression sources we utilize to make recombinant proteins.