PROTEOMIC BIOMARKER PROFILING OF CERVICAL SWABS

宫颈拭子的蛋白质组生物标志物分析

• 批准号: 8361413
• 负责人: Janet S. Rader
• 金额: $ 0.81万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361413
• 关键词: Aryl Hydrocarbon Receptor; Biological Markers; Cell Cycle; Cells; Cervical; Cervical Cancer Screening; Cervix Uteri; Collection; DNA damage checkpoint; Data Set; Development; Epithelial; Funding; Genes; Genomics; Grant; Liquid substance; Malignant neoplasm of cervix uteri; Mass Spectrum Analysis; Methods; Microarray Analysis; Modeling; Molecular; National Center for Research Resources; Nucleic Acids; Outcome; Pathway interactions; Patients; Pattern; Principal Investigator; Proteins; Proteomics; Protocols documentation; Receptor Signaling; Regulation; Research; Research Infrastructure; Resources; Sampling; Screening procedure; Signal Transduction; Source; Specimen; Spottings; Swab; Tissues; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; biomedical resource; cDNA Arrays; cancer cell; clinical practice; cost; endoplasmic reticulum stress; interest; neoplastic; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Rader lab is interested in using integrated proteomic and genomic screening to find potential biomarkers for cervical cancer. Cervical cancer screening is ideally suited for the development of biomarkers due to the ease of tissue acquisition and the well- established histological transitions. Furthermore, cell and biologic fluid obtained from cervix samples undergo specific molecular changes that can be profiled and models constructed that may predict outcome. A patient screening protein and nucleic acid collection protocol was established using standard spatulas and cytobrushes already used in clinical practice. RNAlater was used to collect the samples followed by proteomic methods to identify biomarkers/proteins that were differentially expressed in normal cervical epithelial versus cervical cancer cells. Three hundred ninety spots were identified via two-dimensional difference gel electrophoresis (2-D DIGE) that were expressed at either higher or lower levels (>3-fold) in cervical cancer samples. These proteomic results were compared to the most significant genes in a cDNA microarray analysis of microdissected neoplastic cervical specimens to identify overlapping patterns of expression. The most frequent pathways represented by the combined dataset were: cell cycle: G2/M DNA damage checkpoint regulation; aryl hydrocarbon receptor signaling; p53 signaling; cell cycle: G1/S checkpoint regulation; and the endoplasmic reticulum stress pathway.

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