COOP COLLAPSE OF GROUP IIC INTRON ALONG FOLDING PATHWAY BY METAL IONS&OSMOLYTES

金属离子导致IIC族内含子沿折叠途径的COOP塌陷

• 批准号: 8363552
• 负责人: Anna Marie Pyle
• 金额: $ 0.5万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363552
• 关键词: Architecture; Biochemical; Biological; Characteristics; Coupling; Data; Funding; Grant; Introns; Ions; Measurement; Metal Binding Site; Metals; Methods; Mono-S; NMR Spectroscopy; National Center for Research Resources; Nucleotides; Pathway interactions; Principal Investigator; Process; RNA; RNA Folding; Research; Research Infrastructure; Residual state; Resources; Source; Structure; United States National Institutes of Health; Work; cost; interest; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. RNA is an important and biological relevant molecule, which is involved in a number of critical cellular and regulatory functions. We are interested in studying the folding pathway and structural intermediate(s) of a group IIC intron from O. iheyensis  a ~400 nt RNA. At the moment little is known about the specific features of this RNA molecule, and the structural characteristics that help it to traverse its folding landscape and correctly attain its functional native state. By observing the compaction that results from global structural transitions (Rg and Dmax) that accompany changes in the concentrations of biologically relevant and important mono- and divalent ions, and osmolytes we hope to better understand the architecture of this RNA. Moreover, SAXS data can be readily combined with information from biochemical experiments to yield nucleotide specific information on the sites of metal binding and localization within the RNA, which will illuminate what local structural transitions occur concurrently with global transformations. The specific aims of this research is to establish a better understanding of the general processes of folding and RNA architecture as they relate to basic structural features of RNA. I have a background in utilizing NMR spectroscopy and residual dipolar coupling experiments to study the structure and dynamics of RNA junctions and motifs. I have attended a couple of short SAXS courses in the last year (2-day course by NSLS at BNL, and an 8-day course by EMBL-Hamburg at DESY), and I am currently working on new methods to study the structure and dynamics from analyses of RNA molecules through SAXS measurements and various biochemical footprinting data.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 RNA是一种重要的与生物相关的分子，参与了许多重要的细胞和调节功能。我们感兴趣的是研究来自欧亚大陆的一组IIC内含子的折叠途径和结构中间体(S)。目前，人们对这种RNA分子的具体特征以及帮助其穿越折叠景观并正确达到其功能自然状态的结构特征知之甚少。通过观察伴随着生物相关和重要的单价和二价离子以及渗透分子浓度变化的全局结构转变(Rg和Dmax)所导致的紧凑，我们希望更好地了解该RNA的结构。此外，SAXS数据可以很容易地与生化实验中的信息相结合，产生关于RNA中金属结合和定位位置的核苷酸特定信息，这将说明在全局转化的同时发生了哪些局部结构转变。本研究的具体目的是更好地理解折叠和RNA结构的一般过程，因为它们与RNA的基本结构特征有关。 我有利用核磁共振波谱和剩余偶极耦合实验研究RNA连接和基序的结构和动力学的背景。去年，我参加了几个短期的SAXS课程(BNL的NSLS为期2天的课程，DESY的EMBL-Hamburg为期8天的课程)，目前我正在研究新的方法，通过SAXS测量和各种生化足迹数据对RNA分子进行分析，研究结构和动力学。