INVESTIGATING THE ROLE OF RSC4 ACETYLATION

研究 RSC4 乙酰化的作用

• 批准号: 8363797
• 负责人: GEETA J NARLIKAR
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363797
• 关键词: Acetylation; Biological Process; Chromatin Remodeling Factor; Complex; DNA; DNA Binding; Funding; Gene Mutation; Genes; Genome; Grant; Growth; In Vitro; Mass Spectrum Analysis; Mediating; National Center for Research Resources; Nucleosomes; Organism; Principal Investigator; Process; Property; Regulation; Research; Research Infrastructure; Resources; Role; Source; United States National Institutes of Health; chromatin remodeling; cost; in vivo; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A complex problem for every eukaryotic organism is how to regulate the process of chromatin remodeling, a critical function to mediate DNA accessibility throughout the genome. Using the UCSF Mass Spec Facility, we have found that Rsc4, an essential sub unit of the RSC remodeling complex, is acetylated, by Gcn5, on K25 at its N-terminus. Purification of RSC under standard growth conditions suggests that most of Rsc4 is acetylated. In vitro comparisons of WT and mutant (K25R) RSC complexes reveal similar capabilities to remodel nucleosomes, bind DNA, and recognize acetylation. Since acetylation does not appear to alter the enzymatic properties of RSC, we are taking several in vivo approaches to understand the biological processes in which acetylation is important and to understand the functional consequences of the acetylation. Specifically, we will use mass spectrometry to identify conditions where acetylation is regulated and, combined with genetic mutations, identify the genes responsible for this regulation. These studies are the first to characterize the function of acetylation in the context of a chromatin remodeling complex.

这个子项目是利用这些资源的众多研究子项目之一