LARGE PARTICLE SORTING FOR THE SELECTION OF OPTIMAL APTAMER BINDERS
用于选择最佳适体结合剂的大颗粒分选
基本信息
- 批准号:8361771
- 负责人:
- 金额:$ 1.12万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-04-01 至 2013-03-31
- 项目状态:已结题
- 来源:
- 关键词:AffinityAsthmaBiological MarkersBioterrorismBronchoalveolar LavageCaliberCell modelCollaborationsColorCountryDevelopmentDiagnosticDisease ProgressionDrug DesignEarly DiagnosisFlow CytometryFundingGenerationsGoalsGrantHumanImmune responseInfectionInflammatory ResponseLaboratoriesLibrariesLigandsNational Center for Research ResourcesNational Heart, Lung, and Blood InstituteNational Institute of Allergy and Infectious DiseaseNatural ImmunityParticle SizePerformancePichindePrincipal InvestigatorProcessProgressive DiseaseProteinsProteomeProteomicsResearchResearch InfrastructureResearch PersonnelResourcesRodent ModelSamplingSerumShockSorting - Cell MovementSourceSpeedTechnologyTestingTherapeuticTimeUnited States National Institutes of HealthVirusVirus DiseasesWorkaptamerbasebiodefensebiosafety level 4 facilitybiosignaturechemokinecostcytokinehemorrhagic fever virusimmunological interventionimprovedinstrumentinterestoutcome forecastparticlepathogenprogramsprotein complexprotein expressionprototyperesponsetool
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
While current droplet sorters can be used to sort the particles 20-70 microns in diameter, the size of the particles limit the sort speed to about a thousand per second. As aptamer libraries could contain >109 beads, sorting speed of larger particles will be at a premium. The technologies developed in this grant (Project 2) will result in the development of a high speed sorter (with initial target rates of 100,000 per second) that would greatly assist in sorting one bead one compound aptamer libraries (or library of libraries). This would greatly improve the ability of investigators to select aptamer and thioaptamer high affinity ligands to critical protein targets. The NFCR will develop sorting technologies as described in project 2. The sorting technologies will be integrated into a functional sorter, which will be initially tested at the NFCR to sort aptamer libraries provided by Dr. Gorenstein. Once instrument performance has been determined to be sufficient, a second generation prototype will be assembled in Dr. Gorenstein's laboratory for use by his lab.
Dr. Gorenstein will provide aptamer libraries and protein targets that interest his programs for selection and sorting at the NFCR. Our goal in this project is to utilize our bead-based technology for selecting thioaptamers utilizing multi-color flow cytometry targeting the differentially expressed proteins within the host proteomes in two major projects funded by NIH. In DARPA and more recently an NIAID-funded U01 partnership grant focused on biodefense, Dr. Gorenstein (PI) is developing a new thioaptamer proteomics chip technology to investigate protein expression associated with pathogen-induced inflammatory responses. He will utilize the NFCR facility to select thioaptamers targeting specific cytokines and chemokines and other identified proteins to dissect immune responses of selected viruses of concern in bioterrorism by analyzing the dynamic changes in protein expression. Changes in protein expression are being determined in human cell models as well as rodent models of immune responses to arena viruses such as Lassa and Pichinde (hemorrhagic fever viruses.
These results will aid in the design of drugs to inhibit the identified protein interactions underlying immune response processes and so ameliorate cytopathological immune responses resulting in shock or to enhance "innate immunity" to help mount effective immune response. Elucidating these protein expression changes will allow early diagnosis and enhanced prognosis of viral disease, and subsequent development of effective pharmacological and immunological interventions. UTMB has a BSL-4 facility for handling these select agents such as Lassa and is constructing one of two National Biocontainment Laboratories and this project is in collaboration with a number of noted virologists at UTMB such as Drs. C. J. Peters, Alan Barrett, Judy Aronson and Norbert Herzog. Dr. Gorenstein will also use the NFCR facility for selecting thioaptamers (again those involved in the immune response) to develop a thioaptamer proteomics chip as a proteomics biomarker/biosignature diagnostic tool to identify and quantify the differential expression of key proteins involved in RSV-infections related to asthma in an NHLBI-funded Proteomics Center at UTMB (one of ten in the country). The working hypothesis is that by utilizing the massive proteome HTS shown in the figure, a limited range of proteins in serum and lung lavage samples can be targeted. Accomplishing this will provide this program the opportunity to construct a biosignature "chip" which will allow identification and quantification of the various important proteins expressed during disease progression. In both projects, the long-term objectives are to develop a real-time thioaptamer chip-based identification and readout of the levels of proteins and protein+protein complexes to allow these researchers to correlate changes in protein expression to predict biomarkers/biosignatures for progressive disease and therapeutic responses. Dr. Gorenstein's lab will serve as a beta testing facility for new high speed large particle sorters and will communicate with the NFCR to optimize instrument performance.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
David G Gorenstein其他文献
David G Gorenstein的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('David G Gorenstein', 18)}}的其他基金
LARGE PARTICLE SORTING FOR THE SELECTION OF OPTIMAL APTAMER BINDERS
用于选择最佳适体结合剂的大颗粒分选
- 批准号:
8169407 - 财政年份:2010
- 资助金额:
$ 1.12万 - 项目类别:
LARGE PARTICLE SORTING FOR THE SELECTION OF OPTIMAL APTAMER BINDERS
用于选择最佳适体结合剂的大颗粒分选
- 批准号:
7956790 - 财政年份:2009
- 资助金额:
$ 1.12万 - 项目类别:
LARGE PARTICLE SORTING FOR THE SELECTION OF OPTIMAL APTAMER BINDERS
用于选择最佳适体结合剂的大颗粒分选
- 批准号:
7724269 - 财政年份:2008
- 资助金额:
$ 1.12万 - 项目类别:
Role of Nitric Oxide and Cyclic GMP in Stem Cells
一氧化氮和环鸟苷酸在干细胞中的作用
- 批准号:
7623532 - 财政年份:2007
- 资助金额:
$ 1.12万 - 项目类别:
Role of Nitric Oxide and Cyclic GMP in Stem Cells
一氧化氮和环鸟苷酸在干细胞中的作用
- 批准号:
7872757 - 财政年份:2007
- 资助金额:
$ 1.12万 - 项目类别:
Combinatorial Selection of Beta-Catenin/T Cell Factor Pathway Inhibitors
β-连环蛋白/T 细胞因子通路抑制剂的组合选择
- 批准号:
7279882 - 财政年份:2006
- 资助金额:
$ 1.12万 - 项目类别:
Computational and Structural Biology in Biodefense
生物防御中的计算和结构生物学
- 批准号:
7274687 - 财政年份:2005
- 资助金额:
$ 1.12万 - 项目类别:
Computational and Structural Biology in Biodefense
生物防御中的计算和结构生物学
- 批准号:
6949320 - 财政年份:2005
- 资助金额:
$ 1.12万 - 项目类别:
Computational and Structural Biology in Biodefense
生物防御中的计算和结构生物学
- 批准号:
7112261 - 财政年份:2005
- 资助金额:
$ 1.12万 - 项目类别:
相似海外基金
Defining new asthma phenotypes using high-dimensional data
使用高维数据定义新的哮喘表型
- 批准号:
2901112 - 财政年份:2024
- 资助金额:
$ 1.12万 - 项目类别:
Studentship
Basophilic oncostatin M fuels nociceptor neuron-induced asthma
嗜碱性制瘤素 M 促进伤害感受器神经元诱发哮喘
- 批准号:
485504 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Salary Programs
Engaging Patient and Caregivers in Using Patient-reported Outcomes Measures in Pediatric Clinical Care for Asthma
让患者和护理人员参与儿科哮喘儿科临床护理中患者报告的结果测量
- 批准号:
495593 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Air pollution and Asthma in Canada: Projections of burden and the value of climate adaptation strategies
加拿大的空气污染和哮喘:负担预测和气候适应战略的价值
- 批准号:
485322 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Operating Grants
Data-driven model links BMIz to gene expression in pediatric asthma
数据驱动模型将 BMIz 与小儿哮喘基因表达联系起来
- 批准号:
493135 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
BIOlogic drug safety and effectiveness interNational pharmacoepidemiologIC study in pregnant women with autoimmune disorders and asthma and their children (BIONIC)
患有自身免疫性疾病和哮喘的孕妇及其子女的生物药物安全性和有效性国际药物流行病学研究(BIONIC)
- 批准号:
493526 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Operating Grants
A Novel Approach to Target Neutrophilic Airway Inflammation and Airway Hyperresponsiveness in Therapy-Resistant (Refractory) Asthma.
一种针对难治性哮喘中性粒细胞性气道炎症和气道高反应性的新方法。
- 批准号:
10659658 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Treating Maternal Depression in an Urban Community-Based Pediatric Asthma Clinic: Targeting Maternal Mood, Child Asthma Outcomes, and Health Disparities
在城市社区小儿哮喘诊所治疗孕产妇抑郁症:针对孕产妇情绪、儿童哮喘结果和健康差异
- 批准号:
10723233 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Improving Prediction of Asthma-related Outcomes with Genetic Ancestry-informed Lung Function Equations
利用遗传祖先信息的肺功能方程改善哮喘相关结果的预测
- 批准号:
10723861 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别:
Prenatal Fatty Acid Supplementation and Early Childhood Asthma and Atopy in Black American Families
美国黑人家庭产前脂肪酸补充剂与儿童早期哮喘和特应性
- 批准号:
10586398 - 财政年份:2023
- 资助金额:
$ 1.12万 - 项目类别: