PROBING THE LIGAND INDUCED CONFORMATIONAL CHANGE OF BETA-PHOSPHOGLUCOMUTASE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 拟议的实验旨在确定导致先前通过X射线结晶学观察到的β-磷酸葡萄糖变位酶(BPGM)构象变化的主要蛋白质-配体相互作用。BPGM是广泛存在的卤代烷酸脱卤酶超家族(HADSF)中最大的亚家族的一部分，该家族在自然界中执行大量的磷酰化转移。识别导致构象变化的关键配体-蛋白质相互作用将有助于深入了解这种转变的机制和底物特异性在这个亚家族中的进化。这些研究也将为利用SAXS研究蛋白质-配体相互作用提供一个有用的模型系统。要求快速查阅，以便进行初步数据收集，以便制定一份完整的提案，并为这一领域的筹资机会提供初步结果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proposed experiments aim to identify the major protein-ligand interactions that induce the conformational change previously observed by X-ray crystallography in beta-phosphoglucomutase (bPGM). bPGM is part of the largest subfamily of the ubiquitous Haloalkanoate Dehalogenase Superfamily (HADSF) that performs the bulk of phosphoryl transfer in nature. Identification of the crucial ligand-protein interactions responsible for the conformational change will give insight into the mechanism of this transition and the evolution of substrate specificity in this subfamily. These studies will also provide a useful model system for the study of protein-ligand interactions via SAXS. Rapid access is requested to allow for initial data collection towards a full proposal as well as to provide preliminary results for funding opportunities in this area.