PROTEINS IN MALARIAL AND TUBERCULOSIS INFECTIONS AND IN BACTERIAL ACQUIRED IMMUN

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Bosch实验室对参与寄生虫进入或离开人类肝脏，红细胞以及Moscovirus载体中的各种细胞类型的疟疾蛋白质感兴趣。我们的目标是通过基于结构的药物设计结合X射线晶体学，通过关键蛋白质的小分子抑制剂来削弱寄生虫的增殖。Prigge实验室对疟疾和结核病关键蛋白的结构生物学感兴趣。 目前的蛋白质晶体包括与结核病的病原体持久性和疟疾的性别分化有关的蛋白质。Bailey实验室对原核生物中新特征的获得性免疫系统进行结构功能研究，该系统与真核生物RNAi的一些基本原理相同。通过对晶体结构和功能数据的分析，我们希望能够辨别这种免疫力的分子机制。为此，我们已经获得了几种相关蛋白质的晶体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Bosch lab is interested in malarial proteins involved in invasion and egress of the parasite into or from various cell types in the human liver, red blood cells as well as in the Mosquito vector. We aim at impairing the parasite's proliferation through small molecule inhibitors for key proteins through structure based drug design in conjunction with x-ray crystallography. The Prigge lab is interested in the structural biology of key proteins from malaria and tuberculosis. Current protein crystals include proteins involved in pathogen persistence in tuberculosis and sexual differentiation in malaria. The Bailey lab performs structure function studies of a newly characterized acquired immune system in prokaryotes, which shares some of the basic principles of eukaryotic RNAi. Through the analysis of crystal structures and functional data we hope to discern the molecular mechanisms underpinning this immunity. To this end we have obtained crystals of several of the proteins involved.