PICOSECOND TIME-RESOLVED LAUE CRYSTALLOGRAPHY

皮秒时间分辨劳厄晶体学

基本信息

  • 批准号:
    8363673
  • 负责人:
  • 金额:
    $ 5.47万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2011
  • 资助国家:
    美国
  • 起止时间:
    2011-08-01 至 2012-07-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We aim to employ the technique of picosecond time-resolved Laue crystallography to study biophysical processes in proteins. Specifically we will use intense short-duration laser pulses to trigger a structural change in a protein crystal and will probe its structural evolution with single X-ray pulses isolated from the synchrotron pulse train using a sequence of X-ray shutters. By acquiring diffraction data at a well-defined instant in time after laser photolysis we can construct a snapshot of the proteins structure with time resolution limited only by the duration of the X-ray pulse which is of the order of 100 ps. We aim to address numerous biophysical questions including the functional role of highly conserved residues in proteins pathways for ligand migration to and from the proteins active site and the correlated structural changes that mediate or control allosteric regulation. To obtain the highest quality data possible we will focus initially on protein systems whose structural changes are reversible. Ligand-binding heme proteins including myoglobin and hemoglobin are ideal model systems for these biophysical investigations. When CO is used as a surrogate for O2 the ligand can be photodissociated from the heme with high quantum efficiency thereby triggering a sequence of events that can be studies structurally. Because the dissociated ligands rebind to the heme bimolecularly on the ms time scale the structure returns to its starting state and the process can be repeated thousands of times. Thus these model systems are ideal for pursuing these biophysical studies.
这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的, 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量, 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的目标是采用皮秒时间分辨劳厄晶体学技术来研究蛋白质的生物物理过程。具体来说,我们将使用强烈的短时间激光脉冲来触发蛋白质晶体的结构变化,并将使用一系列X射线快门从同步加速器脉冲序列中分离出单个X射线脉冲来探测其结构演变。通过在激光光解后的一个明确定义的时刻获取衍射数据,我们可以构建蛋白质结构的快照,其时间分辨率仅受X射线脉冲持续时间的限制,该脉冲的持续时间为100 ps。我们的目标是解决许多生物物理问题,包括高度保守的残基在蛋白质途径中的功能作用,配体迁移到蛋白质的活性位点和相关的结构变化,介导或控制变构调节。为了获得尽可能高质量的数据,我们将首先关注结构变化可逆的蛋白质系统。配体结合血红素蛋白包括肌红蛋白和血红蛋白是这些生物物理研究的理想模型系统。当CO被用作O2的替代物时,配体可以以高量子效率从血红素光解离,从而触发可以在结构上进行研究的一系列事件。因为解离的配体在ms时间尺度上双分子地重新结合到血红素,所以结构返回到其起始状态,并且该过程可以重复数千次。因此,这些模型系统是进行这些生物物理研究的理想选择。

项目成果

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Philip Anfinrud其他文献

Philip Anfinrud的其他文献

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{{ truncateString('Philip Anfinrud', 18)}}的其他基金

PICOSECOND TIME-RESOLVED WAXS OF PROTEINS IN SOLUTION
溶液中的皮秒时间分辨蛋白质蜡
  • 批准号:
    8363675
  • 财政年份:
    2011
  • 资助金额:
    $ 5.47万
  • 项目类别:
PICOSECOND TIME-RESOLVED WAXS OF PROTEINS IN SOLUTION
溶液中的皮秒时间分辨蛋白质蜡
  • 批准号:
    8172009
  • 财政年份:
    2010
  • 资助金额:
    $ 5.47万
  • 项目类别:
PICOSECOND TIME-RESOLVED LAUE CRYSTALLOGRAPHY
皮秒时间分辨劳厄晶体学
  • 批准号:
    8172006
  • 财政年份:
    2010
  • 资助金额:
    $ 5.47万
  • 项目类别:
TIME-RESOLVED WAXS STUDY OF THE T/R TRANSITION OF HEMOGLOBIN
血红蛋白 T/R 转变的时间分辨蜡研究
  • 批准号:
    8172007
  • 财政年份:
    2010
  • 资助金额:
    $ 5.47万
  • 项目类别:
PICOSECOND TIME-RESOLVED CRYSTALLOGRAPHY
皮秒时间分辨晶体学
  • 批准号:
    8172010
  • 财政年份:
    2010
  • 资助金额:
    $ 5.47万
  • 项目类别:
TIME-RESOLVED WAXS STUDY OF THE T/R TRANSITION OF HEMOGLOBIN
血红蛋白 T/R 转变的时间分辨蜡研究
  • 批准号:
    7956818
  • 财政年份:
    2009
  • 资助金额:
    $ 5.47万
  • 项目类别:
PICOSECOND TIME-RESOLVED CRYSTALLOGRAPHY
皮秒时间分辨晶体学
  • 批准号:
    7956816
  • 财政年份:
    2009
  • 资助金额:
    $ 5.47万
  • 项目类别:
PICOSECOND TIME-RESOLVED CRYSTALLOGRAPHY
皮秒时间分辨晶体学
  • 批准号:
    7726025
  • 财政年份:
    2008
  • 资助金额:
    $ 5.47万
  • 项目类别:
TIME-RESOLVED WAXS STUDY OF THE T/R TRANSITION OF HEMOGLOBIN
血红蛋白 T/R 转变的时间分辨蜡研究
  • 批准号:
    7726026
  • 财政年份:
    2008
  • 资助金额:
    $ 5.47万
  • 项目类别:
Developing Picosecond Time-Resolved X-ray Infrastructure at the APS
在 APS 开发皮秒时间分辨 X 射线基础设施
  • 批准号:
    8553425
  • 财政年份:
  • 资助金额:
    $ 5.47万
  • 项目类别:

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