3HE PULMONARY VENTILATION IMAGING OF MICE EXPOSED TO OZONE

3HE 暴露于臭氧的小鼠的肺通气成像

• 批准号: 8363225
• 负责人: John Nouls
• 金额: $ 1.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363225
• 关键词: 3-Dimensional; Air Pollutants; Animal Model; Animals; Breathing; Cardiovascular system; Data; Defect; Distal; Environmental air flow; Fostering; Funding; Gases; Goals; Grant; Health; Heterogeneity; Hospitalization; Hour; Human; Image; Inbred BALB C Mice; Lead; Location; Lower respiratory tract structure; Lung; Magnetic Resonance Imaging; Metric; Microscopy; Mus; National Center for Research Resources; Ozone; Physiological; Principal Investigator; Program Research Project Grants; Pulmonary Ventilation; Research; Research Infrastructure; Resources; Source; Testing; Tissues; United States National Institutes of Health; cost; imaging modality; in vivo; interest; mortality; mouse model; novel; ozone exposure; respiratory; response; software development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mike Foster is a valued collaborator who has supplied us with all necessary mouse models needed to test novel imaging methods. His long-standing interest is in the effect of ozone on a variety of pulmonary regulatory mechanisms detailed below. He seeks to obtain pilot data for 3He MRI in ozone-exposed mice so that HP gas MRI can be included as a possible core in a program project grant application that he is planning to put together in the next year. This would lead to considerable sustained funding for small-animal HP gas MRI as a supporting core. Ambient ozone exposure it associated with increased hospitalizations, respiratory illness, and increased cardiovascular mortality. Understanding the biologic mechanisms that regulate the patho-physiologic responses to common urban air pollutants is of considerable interest to human health. Both animal models and humans have shown that the lower respiratory tract is vulnerable to inhalation of ozone; however there is little information known in vivo of the 3-dimensional inhomogeneity within ventilation units serving the distal parenchymal tissues, which apparently persists three to thirty hours post-exposure. The goals of this project are to: a) quantify the ventilation change between control and ozone-exposed mice, specifically the volume of ventilation defects 24 hours post-exposure; b) identify the physical location of ventilation defects within the lungs; c) develop a metric of heterogeneity of the distribution of ventilation defects within the lungs. To achieve those goals, we will acquire one three-dimensional 3He image (120 ml 3He) of each of 4 control BALB/c mice, as well as 4 animals exposed to ozone. Software developed at the center will be used to quantify the volume of ventilation defects and visualize the location of the defects, and expanded to provide a metric of heterogeneity.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Mike Foster是一位有价值的合作者，他为我们提供了测试新成像方法所需的所有必要小鼠模型。他长期以来的兴趣是臭氧对下文详细介绍的各种肺部调节机制的影响。他试图获得臭氧暴露小鼠的3 He MRI的试验数据，以便HP气体MRI可以作为他计划在明年提交的项目资助申请的可能核心。这将为小动物HP气体MRI提供大量持续资金，作为支持核心。 环境臭氧暴露与住院率、呼吸道疾病和心血管死亡率的增加有关。了解调节常见城市空气污染物的病理生理反应的生物学机制对人类健康具有相当大的意义。动物模型和人类都表明，下呼吸道易受臭氧吸入的影响;然而，对于远端实质组织的通气单元内的三维不均匀性，体内已知的信息很少，这种不均匀性在暴露后明显持续3至30小时。 该项目的目标是： a）量化对照小鼠和臭氧暴露小鼠之间的通气变化，特别是暴露后24小时通气缺陷的体积; B）识别肺内通气缺陷的物理位置; c）开发肺内通气缺陷分布的异质性的度量。 为了实现这些目标，我们将获得一个三维3 He图像（120毫升3 He）的4个控制BALB/c小鼠，以及4只动物暴露于臭氧。该中心开发的软件将用于量化通风缺陷的体积并可视化缺陷的位置，并扩展以提供异质性的度量。