CELLULAR DELIVERY OF RAT INTERVERTEBRAL DISC CELLS IN DISC DEGENERATION MODEL

椎间盘退变模型中大鼠椎间盘细胞的细胞递送

• 批准号: 8363214
• 负责人: Lori A. Setton
• 金额: $ 0.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363214
• 关键词: Cells; Funding; Goals; Grant; Hydrogels; Image; Implant; Intervertebral disc structure; Label; Laboratories; Life; Location; Methods; Microscopy; Modeling; National Center for Research Resources; Optics; Pattern; Principal Investigator; Rattus; Research; Research Infrastructure; Resources; Source; Tail; Time; United States National Institutes of Health; base; cost; improved; intervertebral disk degeneration; nanoparticle; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to investigate the delivery of rat intervertebral disc (IVD) cells in a rat model of disc degeneration. The cells will be delivered to live rat tails (IVD) percutaneously. The cells will be labelled with a fluorescent marker (nanoparticles, Kodak optical imager) to allow us to define the specific location of the implanted cells. We also aim to define the migratory patterns of the injected cells to assist in developing improved methods of cell delivery to IVD. The ability to track implanted cells over a time course will be a significant addition to ongoing efforts in improving cell based delivery methods. Cells may be delivered alone or with a cell delivery vehicle (hydrogel scaffold) developed in our laboratory to maintain the implanted cells in the desired locations. We will plan a longitutional imaging study where the implanted cells will be imaged at 24hrs, 3 days, 7 days and 14 days to determine both the duration of labeling as well as migratory patterns of implanted cells.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们的目标是研究大鼠椎间盘（IVD）细胞在大鼠椎间盘退变模型中的传递。这些细胞将经皮递送至活鼠尾部（IVD）。 这些细胞将用荧光标记（纳米粒子，柯达光学成像仪）进行标记，以便我们确定植入细胞的具体位置。我们还旨在确定注射细胞的迁移模式，以协助开发改进的 IVD 细胞递送方法。在一段时间内跟踪植入细胞的能力将是对改进基于细胞的递送方法的持续努力的重要补充。 细胞可以单独递送，也可以与我们实验室开发的细胞递送载体（水凝胶支架）一起递送，以将植入的细胞维持在所需位置。我们将计划一项纵向成像研究，其中植入的细胞将在 24 小时、3 天、7 天和 14 天进行成像，以确定标记的持续时间以及植入细胞的迁移模式。