GLYCOSPHINGOLIPID COMPOSITIONS DURING INFECTION BY HIV TYPE 1 (HIV-1) PARTICLES

HIV 1型(HIV-1)颗粒感染期间的糖鞘脂组合物

• 批准号: 8365588
• 负责人: SURYARAM GUMMULURU
• 金额: $ 0.85万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365588
• 关键词: Antiviral Therapy; Biology; Coupled; Development; Devices; Electrospray Ionization; Fourier Transform; Funding; Glycosphingolipids; Grant; HIV; HIV Infections; Liquid Chromatography; Mass Spectrum Analysis; Medicine; Membrane; Methods; National Center for Research Resources; Pathway interactions; Performance; Principal Investigator; Research; Research Infrastructure; Resources; Role; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thin Layer Chromatography; United States National Institutes of Health; Virus; cellular targeting; cost; novel; particle; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Previously, Dr. Gummuluru's group obtained initial evidence for the role of HIV-1 particle membrane-associated glycosphingolipids (GSLs) in virus invasion of DCs and also provided information on additional novel cellular targets, GSL biosynthetic pathways and GSL-dependent HIV-1 interactions with DCs, for development of antiviral therapy (Hatch et al., J. Virol. 83:3496, 2009). GSLs are being analyzed by methods currently in use in the Resource, including thin-layer chromatography coupled directly with vibrational cooling matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (TLC-MALDI FTMS) and ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI MS/MS). For this and related projects, the Resource is also exploring the use of a commercial device for direct elution of analytes from TLC plates into nanoESI-MS analysis

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 此前，Gummuluru博士的团队获得了HIV-1颗粒膜相关糖鞘糖脂(GSLs)在DC病毒入侵中作用的初步证据，并提供了关于其他新的细胞靶点、GSL生物合成途径以及依赖GSL的HIV-1与DC相互作用的信息，用于开发抗病毒疗法(Hatch等人，J.Virol)。83：3496,2009)。资源中目前正在使用的分析方法包括直接与振动冷却耦合的薄层色谱-基质辅助激光解吸/电离傅立叶变换质谱仪(TLC-MALDI FTMS)和超高效液相色谱-电喷雾电离串联质谱仪(UPLC-ESI MS/MS)。对于这一项目和相关项目，资源还在探索使用一种商业设备将分析物从薄层色谱板直接洗脱到纳米ESI-MS分析中