DEVELOPMENT OF MOLECULAR LABELING TECHNOLOGIES FOR SOFT X-RAY TOMOGRAPHY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这个核心的主要目标是开发蛋白质定位方法用于 软X射线层析成像。我们将通过进一步发展两个项目来实现这一目标 我们开创了标记和可视化细胞中分子的方法，1)多- 使用荧光标记的模式成像，例如GFP，以及2)使用电子密度 可以在软X射线图像中直接看到的标签。的一个关键组件 多模式方法是使用大口径低温荧光显微镜，一种 NCXT开发的新技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core has the principle goal of developing protein localization methods for use with soft x-ray tomographic imaging. We will achieve this goal by further developing two approaches we have pioneered for labeling and visualizing molecules in cells, 1) Multi- modal imaging using fluorescence labels, such as GFP, and 2) using electron dense labels that can be seen directly in the soft x-ray image. A pivotal component of the multimodal approach is the use of high aperture cryogenic fluorescence microscopy, a new technology developed by the NCXT.