CORE RESEARCH PROJECT 2: CRYO-TILT STAGES FOR TOMOGRAPHY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 除了显微镜本身，要建造的最关键的仪器是为生物样本成像的阶段。这些设备必须在成像过程中实现低温稳定样品的精确计算机控制的平移和旋转。工作台还必须与用于快速冻结样品的仪器相连接，并允许以简单的密封方式将冻结的样品转移到X射线显微镜。这将需要两个不同的阶段：一个阶段用于圆柱形样品，第二个阶段用于安装在平面衬底上的平板样品。我们将首先将注意力集中在圆柱形样本的阶段，因为已经构建了一个功能相当有效的原理证明原型阶段。然后我们将开始开发扁平样品的低温阶段。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The most critical instruments to be constructed, other than the microscope itself, are the stages for imaging biological samples. The devices must achieve precise computer controlled translation and rotation of the cryogenically stabilized sample during imaging. The stages must also interface with instrumentation used to rapidly freeze the samples and allow transfer of the frozen sample to the x-ray microscope in a simple hermetic fashion. This will require two separate stages: one for cylindrical samples and a second stage for flat samples mounted on planar substrates. We will focus our attention on the stage for cylindrical samples first, since a proof-of-principle prototype stage that functions quite efficiently has been constructed. We will then begin development of the flat specimen cryostage.