RNA INTERFERENCE IN THE HUMAN FUNGAL PATHOGEN CRYPTOCOCCUS NEOFORMANS

人类病原体新型隐球菌中的 RNA 干扰

• 批准号: 8365901
• 负责人: Hiten D Madhani
• 金额: $ 2.27万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365901
• 关键词: Basidiomycota; Biological Models; Biology; Body Temperature; Cell Wall; Complement; Complex; Cryptococcus; Cryptococcus neoformans; Funding; Fungal Genome; Gene Expression; Gene Expression Regulation; Genetic; Grant; HIV; Human; Human body; Infection; Melanins; Mycoses; National Center for Research Resources; Organism; Pathogenesis; Patients; Polysaccharides; Principal Investigator; Production; Property; Proteins; RNA Interference; Research; Research Infrastructure; Resources; Role; Source; United States National Institutes of Health; Untranslated RNA; capsule; cost; fungus; pathogen; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The basidiomycete Cryptococcus neoformans is responsible for the most common systemic fungal infection in HIV patients. This infection depends upon several properties of the fungus, including its ability to grow at human body temperature, its polysaccharide capsule, and its production of cell wall-associated melanin, each of which is controlled by complex gene expression programs. We seek to explore the role of RNA interference in Cryptococcus gene expression regulation. Interestingly, although RNAi is known to be active in Cryptococcus, its complement of RNAi proteins appears to be distinctively diversified as compared to other basidiomycetes. Thus, characterization of the mechanism of RNAi and its genetic targets in this organism could both further our understanding of Cryptococcus pathogenesis and also provide a unique new model system for the study of RNA interference and noncoding RNA.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 新型隐球菌是引起HIV患者最常见的系统性真菌感染的主要原因。 这种感染取决于真菌的几种特性，包括其在人体温度下生长的能力，其多糖胶囊，以及其细胞壁相关黑色素的产生，其中每一种都由复杂的基因表达程序控制。 我们试图探索RNA干扰在隐球菌基因表达调控中的作用。 有趣的是，虽然已知RNAi在隐球菌中是有活性的，但与其他担子菌相比，其RNAi蛋白的补体似乎是独特多样的。 因此，对隐球菌RNAi机制及其遗传靶点的研究，不仅可以进一步加深对隐球菌致病机制的认识，而且可以为RNA干扰和非编码RNA的研究提供一个独特的新的模型系统。