TRANSMEMBRANE DOMAIN FROM INFLUENZA HEMAGGLUTININ/MEMBRANE INTERACTIONS

流感血凝素/膜相互作用的跨膜结构域

• 批准号: 8364034
• 负责人: MAOCHEN GE
• 金额: $ 0.16万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364034
• 关键词: Funding; Grant; Hemagglutinin; Influenza Hemagglutinin; Link; Lipids; Mediating; Membrane; Membrane Fusion; National Center for Research Resources; Play; Principal Investigator; Research; Research Infrastructure; Resources; Role; SNAP receptor; Source; Technology; Transmembrane Domain; United States National Institutes of Health; Vesicle; cost; influenzavirus; synthetic peptide; target SNARE proteins; vesicular SNARE proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We initiated a study of lipid interactions of a synthetic peptide corresponding to the transmembrane domain (TM) of hemagglutinin (HA) from influenza virus. We are intrigued by a question of whether the TM plays a passive role, or is actively involved in HA-mediated membrane fusion. Recently it was shown that replacing the TM of HA with a glycophosphoinositol-linked lipid anchor results in hemifusion, but not complete fusion. Also replacing either the v-SNARE or t-SNARE (or both) peptidic anchors with a single lipid unit, prohibits lipid mixing in SNARE-mediated vesicle fusion. It was suggested that simply holding two vesicles together is not sufficient for membrane fusion, and a pulling force exerted on the membrane anchors might be important for the fusion activity.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们启动了一项针对流感病毒血凝素（HA）跨膜结构域（TM）的合成肽的脂质相互作用的研究。我们感兴趣的一个问题，TM是否发挥了被动作用，或积极参与HA介导的膜融合。 最近显示，用糖磷酸肌醇连接的脂质锚代替HA的TM导致半融合，但不完全融合。此外，用单个脂质单元替换v-SNARE或t-SNARE（或两者）肽锚，阻止了SNARE介导的囊泡融合中的脂质混合。有人提出，简单地将两个囊泡保持在一起不足以进行膜融合，并且施加在膜锚上的拉力对于融合活性可能是重要的。