ESR STUDIES OF PHASE STRUCTURES IN DSPC/POPC/CHOL AND DSPC/DOPC/CHOL MIXTURES

DSPC/POPC/CHOL 和 DSPC/DOPC/CHOL 混合物中相结构的 ESR 研究

• 批准号: 8364041
• 负责人: MAOCHEN GE
• 金额: $ 0.05万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364041
• 关键词: Biological; Dimensions; Dyes; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Funding; Grant; Light; Membrane; Membrane Microdomains; National Center for Research Resources; Phase; Phospholipids; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Source; Spin Labels; Structure; Technology; United States National Institutes of Health; Vesicle; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In biological membranes, when lo + ld phases coexist ("rafts"), the size of the domains seems to be much smaller than the wavelength of light, i.e. at least one dimension of size < 300 nm. In chemically-defined mixtures of SM/POPC/chol, or DSPC/POPC/chol, or DSPC/SOPC/chol, giant unilamellar vesicles (GUVs) appear uniform in fluorescence microscopy over the entire composition region where lipid rafts could occur. However, FRET and single dye fluorescence studies indicate phase separation at well-defined boundaries. By use of a spin-labeled phospholipid, "16-PC", we examine the hyperfine splitting of samples across the putative phase boundaries in DSPC/POPC/chol, and compare with the hyperfine splitting across phase boundaries in DSPC/DOPC/chol, where large phase domains are observed in GUVs.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在生物膜中，当lo + ld相共存（“筏”）时，域的尺寸似乎比光的波长小得多，即至少一个尺寸< 300 nm。 在SM/POPC/chol或DSPC/POPC/chol或DSPC/SOPC/chol的化学定义的混合物中，在荧光显微镜下，巨大的单层囊泡（GUV）在可能出现脂筏的整个组合物区域内均匀出现。 然而，FRET和单一染料荧光研究表明在明确定义的边界相分离。 通过使用自旋标记的磷脂，“16-PC”，我们检查了超精细分裂的样品在DSPC/POPC/胆固醇的假定的相边界，并与超精细分裂在DSPC/DOPC/胆固醇，其中大的相域中观察到的GUV跨相边界。