INTERNAL FUSION PEPTIDE OF THE SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们已经启动了一项与 SARS-CoV (IFP18) 内部融合肽相对应的合成肽的脂质相互作用的研究。融合肽可分为 N 端肽或内部肽，具体取决于它们相对于病毒融合蛋白切割位点的位置。已知冠状病毒刺突蛋白 (S) 蛋白在 S1/S2 边界处被切割。该切割位点与融合肽没有紧密连接。据报道，一种由 18 个残基组成的合成肽，紧邻 SARS-CoV (IFP18) 第二个 S2 切割位点的 C 端，可促进脂质混合。诱变研究表明，SARS-CoV 序列中的一些保守残基对于 SARS-Cov 介导的病毒融合至关重要。因此，该肽被认为是内部融合肽[1]。 [1] 马杜，I.G.，S.L.罗斯 (S. Belouzard) 和 G.R.惠特克。 2009.严重急性呼吸综合征冠状病毒刺突蛋白S2结构域内高度保守结构域的表征，具有病毒融合肽的特征。 J.维罗尔。 83：7411-7421。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have initiated a study of lipid interactions of a synthetic peptide corresponding to the internal fusion peptide from SARS-CoV (IFP18). Fusion peptide can be classified as an N-terminal or internal, depending on their location relative to the cleavage site of the viral fusion protein. The coronavirus spike protein (S) protein is known to be cleaved at the S1/S2 boundary. This cleavage site is not closely linked to a fusion peptide. It was reported that a synthetic peptide, consisting of 18 resides, immediately C-terminal to a second S2 cleavage site of SARS-CoV (IFP18), promotes lipid mixing. Mutagenesis studies showed that some conserved residues in this sequence of SARS-CoV are critical in viral fusion mediated by SARS-Cov. Thus, this peptide was suggested to be an internal fusion peptide [1]. [1] Madu, I.G., S.L. Roth, S. Belouzard, and G.R. Whittaker. 2009. Characterization of highly conserved domains within the severe acute respiratory syndrome coronavirus spike protein S2 domain with characteristics of a viral fusion peptide. J. Virol. 83:7411-7421.