INTEGRIN ASSOCIATED PROTEINS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 整合素受体与细胞外基质（ECM）配体结合，并选择性地连接到细胞内信号传导途径和肌动蛋白细胞骨架，以调节粘附依赖性细胞过程。在癌症中，整合素对粘附的动态调节对于细胞附着于ECM、细胞增殖、侵袭和细胞迁移是至关重要的。然而，对于口腔癌迁移、侵袭和转移重要的整合素的细胞内下游效应物尚不清楚。我们的目的是比较整合素相关复合物的组成，来自高转移性和低转移性口腔癌细胞。这项蛋白质组学研究将确定候选整合素下游因子，这些因子与不同的整合素受体相关，或与特定转移状态下的整合素特异性相关。未来的研究将描述是否确定的因素调节侵袭和转移，以及参与调节这些转移表型的分子途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Integrin receptors bind to extracellular matrix (ECM) ligands and selectively connect to intracellular signaling pathways and the actin cytoskeleton to regulate adhesion-dependent cellular processes. In cancer, the dynamic regulation of adhesion by integrins is critical for cell attachment to ECM, cell proliferation, invasion and cell migration. However, the intracellular downstream effectors of integrins that are important for oral cancer migration, invasion and metastasis are not well understood. Our aim is to compare the composition of integrin associated complexes derived from highly and poorly metastatic oral carcinoma cells. This proteomics study will identify candidate integrin downstream factors that either associate with distinct integrin receptors or specifically associate with integrins in a particular metastatic state. Future studies will delineate whether the identified factors regulate invasion and metastasis, as well as the molecular pathways involved in regulating these metastatic phenotypes.