STRUCTURE DETERMINATION OF EBOLA VIRUS VP35 PROTEIN BY PDS

PDS 测定埃博拉病毒 VP35 蛋白的结构

• 批准号: 8364072
• 负责人: ELKA R GEORGIEVA
• 金额: $ 0.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364072
• 关键词: Binding; Biochemical; C-terminal; Complex; DNA-Directed RNA Polymerase; Double-Stranded RNA; Ebola virus; Ebola virus VP35 protein; Funding; Goals; Grant; In Vitro; Interferons; Length; Measurement; Methods; N-terminal; National Center for Research Resources; Physiologic pulse; Principal Investigator; Property; Protein Kinase; Proteins; RNA; RNA Interference; Research; Research Infrastructure; Resources; Source; Structure; Technology; United States National Institutes of Health; Virulence; cost; dimer; human mortality; in vivo; monomer; mutant; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Ebola virus VP35 is a multifunctional protein significant to VP35 virulence and human mortality. It is a part of the viral RNA polymerase complex; also it is responsible for the RNA silencing, suppression and RNA-depended protein kinase inhibition. VP35 is composed of N-terminal coiled-coil region and C-terminal interferon inhibitory domain, which binds dsRNA. Two crystal structures of the C-terminal part of the protein were released in the last two years, with and without bound 8-bp dsRNA. It was found that this domain is monomeric when it is RNA free or else crystallizes as a dimer of dimers when bound to dsRNA. Even though several in vitro as well as in vivo biochemical studies were performed to elucidate the oligomerization states of this protein, there is no structural information on the full length EV VP35. Biochemical methods suggested the existence of at least four oligomeric forms of VP35 (monomer to tetramer, and trimer/tetramer), with some of them responsible for the virulence of the EV. Our goal is to make inquiry into the oligomerization properties of the full length EV VP35 by using pulse dipolar ESR and to follow on to infer its structure with the distance measurements on a set of single and double mutants.

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