PULSED DIPOLAR ESR STUDY ON MEMBRANE OF EBOLA VIRUS FUSION PEPTIDE

埃博拉病毒融合肽膜的脉冲偶极ESR研究

基本信息

  • 批准号:
    8364030
  • 负责人:
  • 金额:
    $ 3.59万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2011
  • 资助国家:
    美国
  • 起止时间:
    2011-09-01 至 2012-08-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Ebola virus (EV) causes hemorrhagic fevers in humans with a high mortality. Although significant research effort have benapplied to understand the mechanism of viral infection, and more specifically the mechanism of viral fusion into the host cell, it remains unrevealed. It is known, to date, that EV shares the common properties of viral fusion: A single surface transmembrane glycoprotein (GP), belonging to class I viral fusion proteins plays a central role in the entry into the target cells by mediating the merging and fusion between the viral and the host cell membranes. The Ebola GP, expressed as a single-chain precursor, is cleaved posttranslational into two disulfide linked fragments GP1 and GP2. A specific feature of EV is the internal fusion peptide (IFP) located in the GP2 domain, containing approximately sixteen uncharged hydrophobic residues (residues 524  539) that is typical for a family of viral fusion peptides. It is thought that in the fusion active state the GP2 protein inserts its intrinsic FP into the membrane of the target cell and this is a critical stage in the fusion process. Despite what is available from the literature, there is no complete understanding of the EV fusion mechanism. Moreover, in contrast to N-terminal fusion peptides, generally the internal fusion peptides are poorly characterized in their interaction with membranes and possible structural role. In regard to above, we have undertaken an in vitro pulsed dipolar ESR study on a synthetic peptide with the amino acid sequence GAAIGLAWIPYFGPAA, representing the naturally accruing Ebola virus fusion region, and two modifications with substituted Ala2Cys or Ala2 to Cys and Ala15to Cys. Mutations to cysteines makes it possible to attach paramagnetic spin-labels to these residues and obtain structural information by measuring distances between these spin-labels. Hence, different conformations upon binding to mimetic membranes could be determined and characterized.
这个子项目是利用这些资源的众多研究子项目之一

项目成果

期刊论文数量(0)
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ELKA R GEORGIEVA其他文献

ELKA R GEORGIEVA的其他文献

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{{ truncateString('ELKA R GEORGIEVA', 18)}}的其他基金

USE OF LIPIDIC NANODISCS FOR STRUCTURE/FUNCTION STUDIES ON MEMBRANE PROTEINS
使用脂质纳米圆盘进行膜蛋白的结构/功能研究
  • 批准号:
    8364070
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
FREEZE-QUENCH STUDY ON PROTEIN CONFORMATION STATE
蛋白质构象状态的冷冻淬灭研究
  • 批准号:
    8364073
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
PROBING ALPHA-SYNUCLEIN AGGREGATION
探测 α-突触核蛋白聚集
  • 批准号:
    8364109
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
PROBING BACTERIAL HOMOLOGUE OF GLUTAMATE TRANSPORTER BY PULSED DIPOLAR ESR
通过脉冲偶极 ESR 探测谷氨酸转运蛋白的细菌同源物
  • 批准号:
    8364071
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
NEW INSIGHTS INTO THE STRUCTURAL PROPERTIES OF ALPHA-SYNUCLEIN AND ITS MUTANTS
对 α-突触核蛋白及其突变体结构特性的新见解
  • 批准号:
    8364031
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
BUILDING UP THE FACILITY FOR MEMBRANE PROTEIN MANIPULATION AND SPIN LABELING
建立膜蛋白操作和旋转标记设施
  • 批准号:
    8364069
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
PULSED DIPOLAR ESR STUDY ON MEMBRANE-BOUND ALPHA-SYNUCLEIN
膜结合 α-突触核蛋白的脉冲偶极 ESR 研究
  • 批准号:
    8364019
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
INCREASING THE DISTANCE RANGE AND RESOLUTION IN PULSED DIPOLAR ESR SPECTROSCOPY
提高脉冲偶极 ESR 光谱的距离范围和分辨率
  • 批准号:
    8364033
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
STRUCTURE DETERMINATION OF EBOLA VIRUS VP35 PROTEIN BY PDS
PDS 测定埃博拉病毒 VP35 蛋白的结构
  • 批准号:
    8364072
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:
PDS STUDY ON HUMAN PGP MDR TRANSPORTER ABCB1
人类 PGP MDR 转运蛋白 ABCB1 的 PDS 研究
  • 批准号:
    8364053
  • 财政年份:
    2011
  • 资助金额:
    $ 3.59万
  • 项目类别:

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