Project 3: Environmentally Persistent Free Radicals in Contaminated Soils

环境持久性自由基（EPFRs）的水平在五氯酚（PCP）污染的土壤中被发现，比在邻近地区的原始土壤样本中高出30倍。这一发现很重要，因为PCP的广泛使用，epfr的潜在毒性，由于从现场吹来的灰尘而可能暴露于这些efpr，它们迁移到地下水并通过地下水以及皮肤暴露。因此，需要对epfr如何在被PCP污染的土壤中形成有一个基本的了解。由于土壤的复杂性，有许多途径可以解释epfr的形成。本项目将通过三个具体目标系统地探索pccp污染土壤中epfr的形成：1)物理分离，化学编辑和表征污染和非污染土壤的有机和无机成分；2)确定污染土壤中导致epfr形成的主要土壤成分；3)利用基于Specific Aim-1研究结果的模型系统来深入了解污染土壤中自由基的自然“原位”形成。这个项目是独一无二的，因为它处理“真实世界”的样本。这意味着该项目将作为项目1中热EPFR形成路径的一些概念的普遍性的试验场，这样做将作为项目1的反馈回路。与项目6的合作将进一步加强这一反馈循环。从Aim-1、Aim-2和Aim-3中选择样本，通过与Materials Core合作提供给生物医学项目2、4和5。除了样品之外，该项目还将提供基本的化学知识，以了解吸入这些含有epfr的样品所引起的任何观察到的心肺功能障碍。

Environmentally persistent free radicals (EPFRs) have been found at levels 30 times higher in the pentachlorophenol (PCP) contaminated soils from a Superfund site - a former wood-treatment facility, than in pristine soil samples from the neighboring area. This finding is important due to the widespread use of PCP, potential of toxicity of EPFRs, potential exposure to these EFPRs due to wind blown dust from the site, their migration to and through ground waters as well as dermal exposure. Thus, a fundamental understanding of how the EPFRs can be formed in soils that have been contaminated with PCP is needed. Due to the complexity of soils, a number of pathways could account for the formation of EPFRs. This project will systematically explore the formation of EPFRs within PCP-contaminated soils through three Specific Aims: 1) Physically separate, chemically edit and characterize the organic and inorganic components of both contaminated and non-contaminated soils; 2) Determine the main soil component(s) responsible for the formation of EPFRs in the contaminated soil; and 3) Utilize model systems based on the findings from Specific Aim-1 to gain insight into the natural 'in-situ' formation of radicals in the contaminated soil. This project is unique as it addresses "real world" samples. This means that this project will act as a testing ground for the universality of some of the concepts derived for thermal EPFR formation pathways in Project 1, and in doing so, will act as a feedback loop to Project 1. This feedback loop will be further strengthened by collaborations with Project 6. Select samples from Aim-1, Aim-2, and Aim-3 will be provided to the biomedical Projects 2, 4 and 5 through the collaboration with the Materials Core. In addition to the samples, this project will provide the basic chemistry to understand any observed cardiac and pulmonary dysfunction induced by inhalation of these EPFR-containing samples.