Project 3: Environmentally Persistent Free Radicals in Contaminated Soils

项目 3：受污染土壤中环境持久性自由基

• 批准号: 8659463
• 负责人: Robert Lewis Cook
• 金额: $ 16.62万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8659463
• 关键词: Accounting; Address; Air; Area; Biological; Biological Models; Biota; Breathing; Cardiac; Chemistry; Collaborations; Complex; Dermal; Dust; Electron Transport; Environmental Pollutants; Environmental Wind; Exposure to; Feedback; Free Radical Formation; Free Radicals; Functional disorder; Hazardous Waste; Human; In Situ; Incubated; Ingestion; Lung; Methods; Minerals; Modeling; Molecular; Monitor; Pathway interactions; Pentachlorophenol; Pesticides; Pharmacologic Substance; Phenols; Population; Regulation; Research; Route; Sampling; Site; Soil; Staging; Superfund; System; Testing; Time; Toxic effect; Wood material; analytical method; base; ground water; innovation; insight; knowledge of results; migration; pollutant; soil sampling; superfund site

Environmentally persistent free radicals (EPFRs) have been found at levels 30 times higher in the pentachlorophenol (PCP) contaminated soils from a Superfund site - a former wood-treatment facility, than in pristine soil samples from the neighboring area. This finding is important due to the widespread use of PCP, potential of toxicity of EPFRs, potential exposure to these EFPRs due to wind blown dust from the site, their migration to and through ground waters as well as dermal exposure. Thus, a fundamental understanding of how the EPFRs can be formed in soils that have been contaminated with PCP is needed. Due to the complexity of soils, a number of pathways could account for the formation of EPFRs. This project will systematically explore the formation of EPFRs within PCP-contaminated soils through three Specific Aims: 1) Physically separate, chemically edit and characterize the organic and inorganic components of both contaminated and non-contaminated soils; 2) Determine the main soil component(s) responsible for the formation of EPFRs in the contaminated soil; and 3) Utilize model systems based on the findings from Specific Aim-1 to gain insight into the natural 'in-situ' formation of radicals in the contaminated soil. This project is unique as it addresses "real world" samples. This means that this project will act as a testing ground for the universality of some of the concepts derived for thermal EPFR formation pathways in Project 1, and in doing so, will act as a feedback loop to Project 1. This feedback loop will be further strengthened by collaborations with Project 6. Select samples from Aim-1, Aim-2, and Aim-3 will be provided to the biomedical Projects 2, 4 and 5 through the collaboration with the Materials Core. In addition to the samples, this project will provide the basic chemistry to understand any observed cardiac and pulmonary dysfunction induced by inhalation of these EPFR-containing samples.

环境持久性自由基（EPFRs）的水平高出30倍，在五氯苯酚（PCP）污染的土壤从超级基金网站-前木材处理设施，比原始土壤样本从邻近地区。由于五氯苯酚的广泛使用、环氧乙烷阻燃剂的潜在毒性、从现场吹来的粉尘可能会接触到这些环氧乙烷阻燃剂、它们会迁移到或通过地下沃茨以及皮肤接触，因此这一发现很重要。因此，有必要从根本上了解受五氯苯酚污染的土壤如何形成EPFR。由于土壤的复杂性，许多途径可以解释EPFRs的形成。本项目将通过三个具体目标系统地探索五氯苯酚污染土壤中EPFRs的形成：1）物理分离、化学编辑和表征污染土壤和非污染土壤的有机和无机组分; 2）确定污染土壤中形成EPFRs的主要土壤组分;以及3）利用基于具体目标-1的发现的模型系统，以深入了解受污染土壤中自由基的自然“原位”形成。这个项目是独一无二的，因为它解决了“真实的世界”的样本。这意味着，该项目将作为项目1中热EPFR形成路径的一些概念的通用性的试验场，并在此过程中，将作为项目1的反馈回路。这一反馈循环将通过与项目6的合作得到进一步加强。Aim-1、Aim-2和Aim-3中的选定样品将通过与材料核心的合作提供给生物医学项目2、4和5。除了样本，该项目将提供基本的化学，以了解任何观察到的心脏和肺功能障碍诱导吸入这些含EPFR的样本。